Ribozymes and siRNA protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, c2004. |
| Description: | xv, 623 p. : ill. ; 23 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 252 Methods in molecular biology (Clifton, N.J.) ; v. 252. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5150739 |
Table of Contents:
- Preface
- Contributors
- 1. Ribozyme- and siRNA-Mediated mRNA Degradation: A General Introduction
- 2. Combination of Chemical and Enzymatic RNA Synthesis
- 3. Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
- 4. Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection
- 5. Quantification of Ribozyme Target RNA Using Real-Time PCR
- 6. Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping
- 7. Analysis of Global Conformational Transitions in Ribozymes
- 8. In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR
- 9. Identification of Efficient Cleavage Sites in Long-Target RNAs
- 10. Selection In Vitro of Allosteric Ribozymes
- 11. Regulation of Ribozyme Cleavage Activity by Oligonucleotides
- 12. Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo
- 13. Ribozyme Expression Systems
- 14. Design and Expression of Chimeric U1/Ribozyme Transgenes
- 15. Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases
- 16. Helicase-Attached Novel Hybrid Ribozymes
- 17. Functional Gene Discovery Using Hybrid Ribozyme Libraries
- 18. Maxizyme Technology
- 19. Target-Site Selection for the 10-23 DNAzyme
- 20. In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries
- 21. Nucleic Acid Sequence Analysis Using DNAzymes
- 22. Crystallization of the Hairpin Ribozyme: Illustrative Protocols
- 23. An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes
- 24. Design and Optimization of Sequence-Specific Hairpin Ribozymes
- 25. Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro
- 26. Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction
- 27. Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes
- 28. General Design and Construction of RNase P Ribozymes for Gene-Targeting Application
- 29. In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA
- 30. In Vitro Selection of External Guide Sequences for Directing Human RNase P to Cleave a Target mRNA
- 31. RNase P-Mediated Inhibition of Viral Growth by Exogenous Administration of Short Oligonucleotide External Guide Sequence
- 32. RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus
- 33. Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease
- 34. Potential Design Rules and Enzymatic Synthesis of siRNAs
- 35. RNA Interference (RNAi) With RNase III-Prepared siRNAs
- 36. RNAi Expression Vectors in Mammalian Cells
- 37. Gene-Array Analysis of Glioma Cells After Treatment With Anti-PKC-[alpha] siRNA
- 38. RNAi in Living Mice
- 39. Construction and Transfection of PCR Products Expressing siRNAs or shRNAs in Mammalian Cells
- 40. Systemic Delivery of siRNAs
- 41. Adenovirus-Delivered siRNA
- 42. RNAi Expression Vectors in Plant Cells
- 43. Delivery Agents for Oligonucleotides
- 44. Selection of Peptides for Specific Delivery of Oligonucleotides Into Cancer Cells
- 45. Clinical Gene Therapy Research Utilizing Ribozymes: Application to the Treatment of HIV/AIDS
- 46. Critical Steps in the Implementation of Hematopoietic Progenitor-Cell Gene Therapy Using Ribozyme Vectors: A Laboratory Protocol
- Index
