Protein-protein interactions : methods and applications /
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| Imprint: | Totowa, N.J. : Humana Press, c2004. |
|---|---|
| Description: | xvi, 532 p. : ill. ; 24cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 261 Methods in molecular biology (Clifton, N.J.) ; v. 261. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5157622 |
Table of Contents:
- Preface
- Contributors
- Part I.. Overview
- 1. Structural Basis of Protein--Protein Interactions
- 2. Quantitative Analysis of Protein--Protein Interactions
- Part II.. In Vitro Techniques
- 3. Characterization of Protein--Protein Interactions by Isothermal Titration Calorimetry
- 4. Circular Dichroism Analysis for Protein--Protein Interactions
- 5. Protein--Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
- 6. Measuring Rhodopsin--G-Protein Interactions by Surface Plasmon Resonance
- 7. Using Light Scattering to Determine the Stoichiometry of Protein Complexes
- 8. Sedimentation Equilibrium Studies
- 9. Analysis of Protein--Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
- 10. Fluorescence Gel Retardation Assay to Detect Protein--Protein Interactions
- 11. Fluorescence Polarization Assay to Quantify Protein--Protein Interactions
- 12. Studying Protein--Protein Interactions via Blot Overlay or Far Western Blot
- 13. Glutathione-S-Transferase-Fusion Based Assays for Studying Protein--Protein Interactions
- 14. Affinity Capillary Electrophoresis Analyses of Protein--Protein Interactions in Target-Directed Drug Discovery
- 15. Mapping Protein--Ligand Interactions by Hydroxyl-Radical Protein Footprinting
- 16. Use of Phage Display and Polyvalency to Design Inhibitors of Protein--Protein Interactions
- Part III.. Detecting Protein--Protein Interactions in Heterologous Systems
- 17. A Bacterial Two-Hybrid System Based on Transcriptional Activation
- 18. Using the Yeast Two-Hybrid System to Identify Interacting Proteins
- 19. Analysis of Protein--Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
- 20. The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
- 21. Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
- 22. Mammalian Two-Hybrid Assay for Detecting Protein--Protein Interactions In Vivo
- 23. Co-Immunoprecipitation from Transfected Cells
- Part IV. Probing Protein--Protein Interactions in Living Cells
- 24. Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
- 25. Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
- 26. Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions
- 27. Confocal Microscopy for Intracellular Co-Localization of Proteins
- 28. Mapping Biochemical Networks with Protein-Fragment Complementation Assays
- 29. In Vivo Protein Cross-Linking
- Part V.. Proteomics-Based Approaches
- 30. Computational Prediction of Protein--Protein Interactions
- 31. Affinity Methods for Phosphorylation-Dependent Interactions
- 32. Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes
- 33. Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry
- 34. Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry
- 35. Internet Resources for Studying Protein--Protein Interactions
- Index
