RNA interference (RNAi) : nuts & bolts of RNAi technology /

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Bibliographic Details
Imprint:	[Eagleville, PA] : DNA Press, c2003.
Description:	viii, 236 p. : ill. ; 22 cm.
Language:	English
Series:	The nuts & bolts series Nuts & bolts series.
Subject:	RNA Interference -- physiology. RNA, Small Interfering. Biotechnology -- methods. Gene silencing. RNA.
Format:	Print Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/5415783

Hidden Bibliographic Details
Varying Form of Title:	Ribonucleic acid interference (RNAi)
Other authors / contributors:	Engelke, David R. (David Ross)
ISBN:	0966402782 (alk. paper)
Notes:	Includes bibliographical references and index.

Table of Contents:

Introduction
Section I. RNA Interference as an Experimental Tool in Invertebrates
Chapter 1. Targeted Gene Silencing in Plants Using RNA Interference
Chapter 2. RNAi in Caenorhabditis elegans
Chapter 3. Application and Analysis of RNAi in Drosophila Systems
Section II. Short Interfering RNA as a Tool in Vertebrate Systems: Synthesizing the RNA
Chapter 4. Gene Silencing by Synthetic siRNA Duplexes in Mammalian Cell Culture
Chapter 5. Strategies for Synthesizing Small Interfering RNA (siRNA)
Chapter 6. Generating Long dsRNA, Individual siRNA and siRNA Cocktails In Vitro
Section III. Short Interfering RNA as a Tool in Vertebrate Systems: Applications
Chapter 7. Optimizing High Throughput RNAi-based Assays Using Transient Transfection of Synthetic siRNAs in Cultured Mammalian Cells
Chapter 8. Subcellular Delivery and Detection of Small Interfering RNA
Chapter 9. Downregulation of Cellular Genes by PCR Products Expressing siRNAs Mammalian Cells
Chapter 10. Generating Adenoviruses for shRNA or SiRNA Delivery
Chapter 11. Achieving Stable, Heritable Gene Silencing in the Mouse
Index

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