Epidermal cells : methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2005. |
|---|---|
| Description: | xv, 480 p. : ill. ; 26 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 289 Methods in molecular biology (Clifton, N.J.) ; v. 289. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5534468 |
Table of Contents:
- Preface
- Contributors
- Part I. Keratinocyte and Organ Cultures
- 1. Primary Mouse Keratinocyte Culture
- 2. Serial Cultivation of Primary Adult Murine Keratinocytes
- 3. Keratinocyte Culture in the Absence of Substrate Attachment
- 4. Application of Genetically Modified Feeder Cells for Culture of Keratinocytes
- 5. Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis
- 6. Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo
- 7. In Vitro Fabrication of Engineered Human Skin
- Part II. Epidermal Stem Cells
- 8. In Vivo Labeling and Analysis of Epidermal Stem Cells
- 9. Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice
- 10. FACS Enrichment of Human Keratinocyte Stem Cells
- 11. Isolation, Characterization, and Culture of Epithelial Stem Cells
- 12. Keratin 19 as a Stem Cell Marker In Vivo and In Vitro
- Part III. Analysis of Epidermal Differentiation
- 13. Immunolocalization in the Epidermis
- 14. Epidermal Cell Analysis by RT-PCR
- 15. Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
- 16. Analysis of Early Epidermal Development in Zebrafish
- 17. Analysis of E2F Factors During Epidermal Differentiation
- 18. Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis
- 19. Apoptosis in the Epidermis
- 20. Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
- 21. Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry
- 22. MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
- 23. Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
- 24. Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval
- Part IV. Methods and Approaches for the Analysis for Epidermal Function
- 25. Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods
- 26. Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry
- 27. Fluorimetric DNA Assay of Cell Number
- 28. Keratinocyte Transient Transfections
- 29. Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes
- 30. Gene Targeting by Oligonucleotides in Keratinocytes
- 31. Promoter Analysis in the Human SPRR Gene Family
- 32. Stable Integration of Large PAC Constructs in Keratinocytes
- 33. Targeted Somatic Mutagenesis in the Mouse Epidermis
- 34. Methods to Study Protein-Protein Interactions
- 35. Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes
- 36. Analysis of Tissue-Specific DNA Methylation During Development
- 37. Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
- 38. Methods for Gene Expression Profiling in Dermatology Research Using DermArray Nylon Filter DNA Microarrays
- 39. Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis
- Part V. Transplantation and Gene Therapy
- 40. In Vivo Transplantation of Engineered Human Skin
- 41. Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
- 42. Gene and Stem Cell Therapy of the Hair Follicle
- Index
