DNA viruses : methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2005. |
|---|---|
| Description: | xiv, 498 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 292 Methods in molecular biology (Clifton, N.J.) ; v. 292. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5534469 |
Table of Contents:
- Preface
- Contributors
- Part I. Viral Detection
- 1. Viral Detection
- 2. Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1
- 3. Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR
- 4. Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo
- 5. Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo
- 6. Reporter Cell Lines for the Detection of Herpes Simplex Viruses
- Part II. Virus Structure and Imaging
- 7. Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy
- 8. Studying the Structure of Large Viruses With Multiresolution Imaging
- 9. Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence
- 10. Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy
- 11. FTIR Microscopy Detection of Cells Infected With Viruses
- Part III. Virus Entry
- 12. The JC Virus-Like Particle Overlay Assay
- 13. Analysis of Fusion Using a Virus-Free Cell Fusion Assay
- 14. Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells
- Part IV. Gene Expression
- 15. Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence
- 16. Identification and Characterization of Herpesviral Immediate-Early Genes
- Part V. Replication and Genome Maintenance
- 17. Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids
- 18. DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins
- Part VI. Pathogenesis
- 19. Pre-B-Cell Colony Formation Assay
- 20. Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis
- Part VII. Complex Cell Systems
- 21. Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection
- 22. Human Papillomavirus Type 31 Life Cycle: Methods for Study Using Tissue Culture Models
- Part VIII. Recombinant Genetics
- 23. Molecular Genetics of Herpesviruses: A Recombinant Technology Approach
- 24. Molecular Genetics of DNA Viruses: Recombinant Virus Technology
- 25. Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis
- 26. Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology
- 27. Selective Silencing of Viral Gene E6 and E7 Expression in HPV-Positive Human Cervical Carcinoma Cells Using Small Interfering RNAs
- Part IX. Computation/Systems Biology of Viruses
- 28. Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray: Global Analysis of HSV-2 Transcript Abundance During Productive Infection
- 29. Real-Time Quantitative PCR Analysis of Viral Transcription
- 30. Rapid Screening of Chemical Inhibitors That Block Processive DNA Synthesis of Herpesviruses: Potential Application to High-Throughput Screening
- Index
