Protein-ligand interactions : methods and applications /
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| Imprint: | Totowa, N.J. : Humana Press, c2005. |
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| Description: | xi, 568 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; 305 Methods in molecular biology (Clifton, N.J.) ; 305. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5628317 |
| Summary: | A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology(tm) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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| Physical Description: | xi, 568 p. : ill. ; 24 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 1588293726 |
| ISSN: | 1064-3745 ; |
