RNA silencing : methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2005. |
|---|---|
| Description: | xi, 338 p. : ill. ; 24 ; cm. |
| Language: | English |
| Series: | Methods in molecular biology ; 309 Methods in molecular biology (Clifton, N.J.) ; v. 309. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5670986 |
Table of Contents:
- Preface
- Contributors
- 1. Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method
- 2. Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis
- 3. Preparation and Analysis of Drosha
- 4. RNA Interference in Caenorhabditis elegans
- 5. Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei
- 6. Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing
- 7. Strategies for the Design of Random siRNA Libraries and the Selection of Anti-GFP siRNAs
- 8. Silencing Gene Expression with Dicer-Generated siRNA Pools
- 9. Inhibition of Gene Expression In Vivo Using Multiplex siRNA
- 10. Gene Silencing by a DNA Vector-Based RNAi Technology
- 11. Plasmid-Based RNA Interference: Construction of Small-Hairpin RNA Expression Vectors
- 12. siRNA Delivery In Vivo
- 13. Peptide-Based Strategy for siRNA Delivery into Mammalian Cells
- 14. Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells
- 15. Exon-Specific RNA Interference: A Tool to Determine the Functional Relevance of Proteins Encoded by Alternatively Spliced mRNAs
- 16. Immunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAs
- 17. Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro
- 18. High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes
- 19. Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA
- Index
