Immunohistopathology : a practical approach to diagnosis /
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| Author / Creator: | Elias, Jules M. |
|---|---|
| Imprint: | Chicago : ASCP Press, c1990. |
| Description: | xvi, 526 p. : ill. ; 24 cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5725390 |
Table of Contents:
- Preface
- Dedication
- Abbreviations
- 1. Immunohistochemical Methods
- Role of Immunohistochemistry in Diagnostic Pathology
- Specimen Size and Formaldehyde Fixation
- Processing and Pretreatment
- Principles of Immunohistochemical Techniques
- Tyramide Signal Amplification Technique (TSA)
- Polyclonal or Monoclonal Antibodies
- Intensification of Staining by Repeated Application of Reagents
- Immunostaining of Previously Stained Sections
- Storage of Antiserum
- Penetration of Immunoreagents
- Concentration of Immunologic Reagents
- Choosing an Immunohistochemical Technique
- Conclusions
- References
- 2. Immunohistochemical Identification of the Undifferentiated Tumor and Tumor of Unknown Origin
- The Diagnostic Approach
- Algorithmic Interpretation and the Panel Approach
- Anaplastic Round Cell Tumors in Adults
- Small Round Blue Cell Tumors of Childhood
- Fibroblastic Tumors
- Solitary Fibrous Tumor
- So-Called "Fibrohistiocytic Tumors"
- Tumors of Unknown Origin
- Conclusions
- References
- 3. The Renal Biopsy
- Techniques for the Study of Renal Tissue
- Indications and Contraindications
- Collection and Transportation
- Fixation
- Tissue Processing for Light Microscopy
- Tissue Processing for Electron Microscopy
- Cryotomy
- Sectioning the Paraffin Block
- Sectioning the Plastic Block
- Staining for Light Microscopy Morphological Studies
- Staining of Frozen Sections
- Staining of Paraffin and Plastic Sections
- Renal Biopsy in Transplantation
- Interpretation of Immunofluorescence
- Immunofluorescence Staining Patterns
- Complement and Coagulation Components
- Immune Complex Solubility, Size, Charge, and Avidity
- Theories of Renal Disease
- Conclusions
- References
- 4. The Skin Biopsy
- Specimen Handling
- Comparative Advantages of Immunofluorescence and Immunoperoxidase Techniques
- Staining Procedures
- Studies in Bullous Disorders
- Diagnosis of Malignant Melanoma (MM) in Paraffin Sections
- References
- 5. The Lymph Node Biopsy
- Immunophenotype
- Distinction of Lymphoid Neoplasms
- Tissue Preparation
- Specimen Handling
- Fine Needle Aspiration Biopsy
- Transport to Reference Laboratories
- Frozen Section Fixation
- Sectioning of Frozen Blocks
- Paraffin Section Fixation
- Decalcification
- Processing and Embedding
- Cytochemical Staining
- Enzyme Histochemistry of Frozen, Paraffin, and Plastic Sections
- Immunostaining of Frozen Sections
- Immunostaining of Paraffin Sections
- Malignant Histiocytic Lymphoma
- Hodgkin's Disease
- Use of IHC for Dfferential Diagnosis of Large Cell Lymphomas
- Genotypic Analysis
- Work-up of a Possible Lymphoma Case
- Conclusions
- References
- 6. Morphology, Cytochemistry, and Immunophenotyping of the Acute Leukemias
- Approaches to Classification of Acute Leukemia
- Morphological and Cytochemical Features of Lymphoid Leukemias
- Correlation of Prognosis With FAB Classification
- Specimen Handling
- Surface Marker Methodology
- Immunophenotyping of Leukemic Lymphoblasts
- Immunologic Analysis of B Lineage ALL
- Immunologic Analysis of T Lineage ALL
- Cytochemical and Surface Marker Analysis in Acute Myeloblastic Leukemia
- Acute Undifferentiated Leukemia
- Hybrid Leukemia/Biphenotypic Leukemia
- Chronic Myeloid Leukemia-Blast Crisis
- Advances in Diagnosing Acute Leukemia
- References
- 7. Sex Steroid Receptors and Biological Markers in Hormone-Dependent Tumors
- Risk Factors and Prognosis in Breast Cancer
- Prognostic Value of Hormone Receptors in Breast Carcinoma
- Antiestrogen Therapeutic Modes
- Anatomic Localization of ER and PgR
- Steroid Receptor Detection Methods
- Quantitation of Assays
- Optimizing Measurement of Hormone Receptors
- Enzyme Digestion
- ER-IHC Quality Assurance
- Reliability of Postmastectomy ER/PgR Measurement With DCC Assay
- False-Positive and False-Negative Staining of Paraffin Sections
- Fine Needle Aspiration (FNA) for ER/PgR Measurement
- Imprint Smears
- Steroid Receptors in Uterus and Endometrium Tumors
- Steroid Receptors in Ovarian Tumors
- Steroid Receptors in Prostate Cancer
- ER and PgR: Summary and Future Developments
- Prognostic Value of Other Proteins in Breast Cancer
- Summary
- References
- 8. Non-Radioactive In Situ Hybridization for Viral Detection
- Comparison of Tinctorial Stains, Immunohistochemistry, and In Situ Hybridization
- Preparation of Target DNA
- Denaturation or Melting
- Controls
- Detection of Hybridized Probe
- NISH Detection Choices
- Fluorescence In Situ Hybridization (FISH)
- Polymerase Chain Reaction (PCR)
- References
- 9. Histochemical and Immunohistochemical Analysis of Amyloid
- Histochemical Classification
- Biochemical and Immunohistochemical Classification
- Histochemical Staining of Amyloid
- Pretreatment Steps for Histochemical Stains
- Enzyme Digestion
- Effects of Fixation
- Autoclaving
- Alkaline Guanidine Method
- Silver Impregnation Techniques
- Immunohistochemical Staining
- Immunohistochemical Fixation
- Types of Amyloid Fibril Proteins
- Hereditary Cerebral Hemorrhage (Dutch and Icelandic Types)
- Familial Amyloid Polyneuropathy
- Long-Term Hemodialysis-Associated Amyloidosis
- Cutaneous Amyloidosis
- Amyloid P Component
- Biopsy Techniques
- Fat Aspiration vs Tissue Biopsy Techniques
- Therapy for Amyloidosis
- Combined Approach for Classifying Amyloids
- References
- 10. Mechanics of Fluorescence Microscopy
- Light and Color
- Primary and Secondary Fluorescence
- Image Intensity and Image Contrast
- Wavelengths
- Filter Selection
- Illumination Incident Light Fluorescence Microscopy
- Selection of Objectives
- Fluorescence Fading
- Fluorescence Photography
- Fixation and Immunofluorescence Applications
- Summary
- References
- Appendices
- Introduction
- Appendix I. Heat-Induced Epitope Retrieval Protocols
- Appendix II. Keratin Expression
- Appendix III. Cytokeratin Analysis
- Index
