Yeast protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, c2006. |
| Description: | xi, 392 p. : ill. ; 23 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; 313 Methods in molecular biology (Clifton, N.J.) ; 313. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5750248 |
Table of Contents:
- Basic Investigations in Saccharomyces cerevisiae
- Isolation of Nucleic Acids
- Purification of Yeast Peroxisomes
- Isolation of Yeast Plasma Membranes
- Isolation of Yeast Mitochondria
- Extraction of Yeast Lipids
- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
- Analysis of Yeast Lipids
- Yeast Fluorescence Microscopy
- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe
- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
- Mutagenesis
- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
- Inducible Degron and Its Application to Creating Conditional Mutants
- Synthetic Lethal Screen
- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
- Chromatin Assembly in a Crude Fraction From Yeast Cells
- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning
- Study of Transcriptional Regulation Using a Reporter Gene Assay
- Assessing Telomeric Phenotypes
- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier
- Yeast Two-Hybrid System Screening
- Import of Precursor Proteins Into Isolated Yeast Mitochondria
- Index
