Yeast protocols /

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Bibliographic Details
Edition:2nd ed.
Imprint:Totowa, N.J. : Humana Press, c2006.
Description:xi, 392 p. : ill. ; 23 cm.
Language:English
Series:Methods in molecular biology ; 313
Methods in molecular biology (Clifton, N.J.) ; 313.
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/5750248
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Other authors / contributors:Xiao, Wei, 1957 April 10-
ISBN:1588294374 (alk. paper)
Notes:Includes bibliographical references and index.
Table of Contents:
  • Basic Investigations in Saccharomyces cerevisiae
  • Isolation of Nucleic Acids
  • Purification of Yeast Peroxisomes
  • Isolation of Yeast Plasma Membranes
  • Isolation of Yeast Mitochondria
  • Extraction of Yeast Lipids
  • Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
  • Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
  • Analysis of Yeast Lipids
  • Yeast Fluorescence Microscopy
  • Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe
  • Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
  • Mutagenesis
  • Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
  • Inducible Degron and Its Application to Creating Conditional Mutants
  • Synthetic Lethal Screen
  • Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
  • Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
  • Chromatin Assembly in a Crude Fraction From Yeast Cells
  • Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
  • Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning
  • Study of Transcriptional Regulation Using a Reporter Gene Assay
  • Assessing Telomeric Phenotypes
  • Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
  • Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier
  • Yeast Two-Hybrid System Screening
  • Import of Precursor Proteins Into Isolated Yeast Mitochondria
  • Index