Adrenergic receptor protocols /
Saved in:
| Imprint: | Totowa, N.J. : Humana Press, c2000. |
|---|---|
| Description: | xiv, 577 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 126 Methods in molecular biology (Clifton, N.J.) ; v.126. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845192 |
Table of Contents:
- Preface
- List of Contributors
- Part I. Genetic Analysis
- 1. Construction of Libraries for Isolation of Adrenergic Receptor Genes
- 2. Isolation of Adrenergic Receptor Genes
- 3. Analyses of Adrenergic Receptor Sequences
- 4. Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes
- 5. Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones
- 6. Genetic Polymorphisms of Adrenergic Receptors
- Part II. RNA Analysis
- 7. Northern Blot Analyses Detecting Adrenergic Receptor mRNAs
- 8. Ribonuclease Protection Assay for the Detection of ß 1 -Adrenergic Receptor RNA
- 9. Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions
- 10. Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate
- 11. Primer Extension Methods for Determination of ß 1 -Adrenergic Receptor mRNA Start Sites
- Part III. Expression Analysis
- 12. Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors
- 13. Expression of ß-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells
- 14. Expression of ß-Adrenergic Receptors in
- 15. Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors
- 16. Transient Transfection and Adrenergic Receptor Promoter Analysis
- 17. Antisense RNA/DNA-Based Techniques to Probe Adrenergic Receptor Function
- 18. Targeted Disruption of Adrenergic Receptor Genes
- Part IV. Receptor and Second Messenger Analysis
- 19. Development of Antibodies to Adrenergic Receptors
- 20. Western Blot Detection of Adrenergic Receptors
- 21. UV Crosslinking of Adrenergic Receptors and Ligands: Detection by SDS-Page
- 22. Detection of ß-Adrenergic Receptors by Radioligand Binding
- 23. Assessing Adrenergic Receptor Conformation Using Chemically Reactive Fluorescent Probes
- 24. Biochemical Methods for Detection and Measurement of Cyclic AMP and Adenylyl Cyclase Activity
- 25. Assay of Arachidonic Acid Release Coupled to ¿ 1 - and ¿ 2 -Adrenergic Receptors
- 26. Patch-Clamp Recording Methods for Examining Adrenergic Regulation of Potassium Currents in Ocular Epithelial Cells
- Part V. Transactivator Analysis
- 27. Southwestern Blots for Detection of a DNA Binding Protein Recognizing the ¿ 1B -Adrenergic Receptor Gene Promoter Bin Gao and George Kunos
- 28. DNase I Footprinting Analysis of Transcription Factors Recognizing Adrenergic Receptor Gene Promoter Sequences
- 29. Electrophoretic Mobility Shift Assay for Detection of DNA Binding Proteins Recognizing ß-Adrenergic Receptor Gene Sequences
- 30. Determination of mRNA Stability and Characterization of Proteins Interacting with Adrenergic Receptor mRNAs
- Part VI. Microscopy Analysis
- 31. Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors
- 32. Distribution of ¿ 2A - and ¿ 2C -Adrenergic Receptor Immunoreactivity in the Central Nervous System
- 33. Quantitative Light Microscopic Autoradiography of ¿ 2 -Adrenergic Radioligand Binding Sites
- 34. In Situ Hybridization of Adrenergic Receptor mRNA in Brain
- 35. Use of Electron Microscopy in the Detection of Adrenergic Receptors
- Index
