Calpain methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2000. |
|---|---|
| Description: | xviii, 343 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 144 Methods in molecular biology (Clifton, N.J.) ; v. 144. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845198 |
Table of Contents:
- Preface
- Glossary of Abbreviations
- Contributors
- Part I.. Purification of Components of the Calpain System
- 1. Purification of [mu]-Calpain, m-Calpain, and Calpastain from Animal Tissues
- 2. A Simple Protocol for Separation and Assay of [mu]-Calpain, m-Calpain, and Calpstatin from Small Tissue Samples
- 3. Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms From Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography
- 4. Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
- 5. Affinity Purification of [mu]-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump: Some Studies on Erythrocyte [mu]-Calpain
- 6. Expression of m-Calpain in Escherichia coli
- 7. Purification and Characterization of Crustacean Calpain-like Proteinases
- 8. Drosophila Calpains: Purification of a Calpain-like Enzyme from Fruit Flies, and Expression in Escherichia coli
- 9. Molecular Analysis of p94 and Its Application to Diagnosis of Limb Girdle Muscular Dystrophy Type 2A
- 10. Purification of Recombinant Calpastatin Expressed in Escherichia coli
- 11. Preparation of Calpastatin Samples for Western Blotting
- 12. Isolation and Characterization of Calpain Activator Protein from Bovine Brain
- Part II.. Assays of Calpain
- 13. Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein
- 14. Casein Zymogram Assessment of [mu]-Calpain and m-Calpain Activity After Traumatic Brain Injury in the Rat In Vivo
- 15. Fluorescence Measurements of Ca[superscript 2+] Binding to Domain VI of Calpain
- 16. Kinetic Analysis of Human [mu]-Calpain Autolysis
- 17. A Sensitive and Continuous Fluorometric Activity Assay Using a Natural Substrate: Microtubule-Associated Protein 2
- 18. Measurement of Calpain Activity In Vitro and In Situ Using a Fluorescent Compound and Tau as Substrates
- 19. Localization of Calpain by Immunofluorescence in Adherent Cells
- 20. A Radioimmunologic Technique for Assessing Calpain Activation in Cells
- Part III.. Specific Tissues
- 21. Calpains and Myogenesis
- 22. Calpastain (the Endogenous Calpain Inhibitor) and Membrane Protein Degradation in Cell Fusion
- 23. The Role of Calpain in Neurofilament Protein Degradation Associated with Spinal Cord Injury
- 24. Calpain-Mediated Truncation of Glutamate Ionotropic Receptors: Methods for Studying the Effects of Calpain Activation in Brain Tissue
- 25. Concurrent Assessment of Calpain and Caspase-3 Activity by Means of Western Blots of Protease-Specific Spectrin Breakdown Products
- 26. Rat Renal Proximal Tubules, Hypoxia, Ionomycin, and Calpain
- 27. Calpain Activity in Rat Renal Proximal Tubules: An In Vitro Assay
- 28. Calpain Activity in Rat Renal Proximal Tubules: An In Situ Assay
- 29. Cellular In Vivo Assay of Calpain Activity Using a Fluorescent Substrate: Application to Study of Anoxic Liver Injury
- 30. Calpain Methods in Hepatic Ischemia--Reperfusion Injury
- 31. Myocardial Ischemia--Reperfusion Injury and Proteolysis of Fodrin, Ankyrin, and Calpastatin
- 32. Calpains in the Lens and Cataractogenesis
- Part IV.. Specific Substrates
- 33. Proteolysis of Cortactin by Calpain in Platelets and In Vitro
- 34. Proteolysis of p53 Protein by Ubiquitous Calpains
- 35. Modulation of Calpain-Mediated Protein Kinase C Activation Within Intact Cells
- 36. Strategies for Regulating Calpain Activities in Living Cells
- 37. Assays of Apoptosis
- Index
