Extracellular matrix protocols /
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| Imprint: | Totowa, N.J. : Humana Press, 2000. |
|---|---|
| Description: | xiv, 370 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 139 Methods in molecular biology (Clifton, N.J.) ; v. 139. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845211 |
Table of Contents:
- Preface
- Contributors
- Part I.. Biochemistry of Extracellular Matrix
- 1. Semipermeabilized Cells to Study Procollagen Assembly
- 2. Quantitative Determination of Collagen Crosslinks
- 3. Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells
- 4. Recombinant Collagen Trimers from Insect Cells and Yeast
- 5. Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag
- Part II.. Biophysics of Extracellular Matrix Proteins
- 6. Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy
- 7. Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers
- 8. Confocal-FRAP Analysis of ECM Molecular Interactions
- 9. Electron Cryomicroscopy of Fibrillar Collagens
- 10. Atomic Force Microspopy Measurements of Intermolecular Binding Strength
- 11. ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy
- Part III.. Molecular Biology of Extracellular Matrix Genes
- 12. Screening for Mutations in Cartilage ECM Genes
- 13. Tissue-Specific KO of ECM Proteins
- 14. Homologous Gene Targeting to Study ECM Assembly
- 15. Enhancer Analysis of the [alpha]1(II) and [alpha]2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic Mice
- 16. Retroviral Delivery of ECM Genes to Cells
- Part IV.. Cell Biology of the Extracellular Matrix
- 17. Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates
- 18. Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protein Binding to the ECM
- 19. Measuring Interactions Between ECM and TGF[beta]-Like Proteins
- 20. Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells
- 21. Neuroepithelial Differentiation Induced by ECM Molecules
- 22. Migration Assays for Oligodendrocyte Precursor Cells
- 23. Cell Adhesion Assays
- 24. Tissue Engineering and Cell-Populated Collagen Matrices
- 25. Solid Phase Assays for Studying ECM Protein-Protein Interactions
- 26. Tissue Engineering of Cartilage
- 27. Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
- 28. Fluorescence Assays to Study Cell Adhesion and Migration In Vitro
- 29. Analyzing Cell-ECM Interactions in Adult Mammary Gland by Transplantation of Embryonic Mammary Tissue from Knockout Mice
- Index
