In situ hybridization protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, 2000. |
| Description: | xiii, 343 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 123 Methods in molecular biology (Clifton, N.J.) ; v. 123. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845215 |
Table of Contents:
- pt. 1. Chromosomal techniques
- Preparation of human partial chromosome paints from somatic cell hybrids
- DNA-protein In Situ convisualization for chromosome analysis
- Radioactive In Situ hybridization to animal chromosomes
- Native polytene chromosomes of Drosophila melanogaster for light and electron microscopic observation of the conformation and distribution of molecules
- Released chromatin or DNA fiber perparations for high-resolution fiber FISH
- In Situ hybridization to polytene chromosomes of Drosophila melanogaster and other dipteran species
- In Situ hybridization to polytene chromosomes
- Comparative gene mapping in exotic species using FISH
- pt. 2. Tissue techniques
- In Situ hybridization with nonradiaoactive probes
- Quantitative In Situ hybridization using radioactive probes to study gene expression in heterocellular systems
- In Situ end-labeling of fragmented DNA and the localization of apoptosis
- Combined immunohistochemical labeling and In Situ hybridization to colocalize mRNA and protein in tissue sections
- In Situ hybridization protocols for detection of viral DNA using radioactive and nonradioactive DNA probes
- Signal amplification for DNA and mRNA
- In Situ localization of PCR-amplified DNA and cDNA
- Ultrastructural detection of nucleic acids on thin sections of tissue embedded in hydrophilic resin
- In Situ hybridization for electron microscopy
- In Situ hybridization of whole-mount embryos
- cRNA probes: comparison of isotopic and nonisotopic detection methods
- pt. 3. Applications
- Molecular cytogenetic analysis of sperm from infertile males undergoing intracytoplasmic sperm injection
- Localization of HIV-1 DNA and tumor necrosis factor-[alpha] mRNA in human brain using polymerase chain reaction In Situ hybridization and immunocytochemistry.
