Connexin methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2001. |
|---|---|
| Description: | xiv, 491 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v.154 Methods in molecular biology (Clifton, N.J.) ; v. 154. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845240 |
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| 245 | 0 | 0 | |a Connexin methods and protocols / |c edited by Roberto Bruzzone and Christian Giaume. |
| 260 | |a Totowa, N.J. : |b Humana Press, |c c2001. | ||
| 300 | |a xiv, 491 p. : |b ill. ; |c 24 cm. | ||
| 336 | |a text |b txt |2 rdacontent |0 http://id.loc.gov/vocabulary/contentTypes/txt | ||
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| 490 | 1 | |a Methods in molecular biology ; |v v.154 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |g Part I. |t Tools to Study Connexins -- |g 1. |t Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques / |r Magali Theveniau-Ruissy, Sebastien Alcolea, Irene Marics, Daniel Gros, Antoon F. M. Moorman and Wouter H. Lamers -- |g 2. |t Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions / |r Irene Dunia, Michel Recouvreur, Pierre Nicolas, Nalin M. Kumar, Hans Bloemendal and E. Lucio Benedetti -- |g 3. |t Purification of Gap Junctions / |r Gina E. Sosinsky and Guy A. Perkins -- |g 4. |t Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions / |r Mark Yeager and Vinzenz M. Unger -- |g 5. |t Connexins/Connexons: Cell-Free Expression / |r Matthias M. Falk -- |g 6. |t Biochemical Analysis of Connexon Assembly / |r Judy K. VanSlyke and Linda S. Musil -- |g 7. |t Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells / |r Dale W. Laird, Karen Jordan and Qing Shao -- |g 8. |t Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction / |r Laurent Venance -- |g 9. |t Use of Retroviruses to Express Connexins / |r Jean X. Jiang -- |g 10. |t Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides / |r Colin R. Green, Lee young Law, Jun Sheng Lin and David L. Becker -- |g 11. |t Transfection and Expression of Exogenous Connexins in Mammalian Cells / |r Dieter Manthey and Klaus Willecke -- |g Part II. |t Assays for Function -- |g 12. |t Assaying the Molecular Permeability of Connexin Channels / |r Paolo Meda -- |g 13. |t Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins / |r I. Martha Skerrett, Mary Merritt, Lan Zhou, Hui Zhu, FengLi Cao, Joseph F. Smith and Bruce J. Nicholson -- |g 14. |t Mutagenesis to Study Channel Structure / |r Gerhard Dahl and Arnold Pfahnl -- |g 15. |t Dual Patch Clamp / |r Harold V.M. Van Rijen, Ronald Wilders, Martin B. Rook and Habo J. Jongsma -- |g 16. |t Determining Ionic Permeabilities of Gap Junction Channels / |r Richard D. Veenstra -- |g 17. |t Fluorescence Recovery After Photobleaching / |r Jean Deleze, Bruno Delage, Olfa Hentati-Ksibi, Franck Verrecchia and Jean-Claude Herve -- |g 18. |t Capture of Transjunctional Metabolites / |r Gary S. Goldberg and Paul D. Lampe -- |g Part III. |t Physiology and Biology of Connexins -- |g 19. |t The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes / |r E. Brady Trexler and Vytas K. Verselis -- |g 20. |t Exploring Hemichannel Permeability In Vitro / |r Andrew L. Harris and Carville G. Bevans -- |g 21. |t Inducing De Novo Formation of Gap Juntion Channels / |r Feliksas F. Bukauskas -- |g 22. |t Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain / |r Taufik A. Valiante, Jose L. Perez Velazquez and Peter L. Carlen -- |g 23. |t Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds: A Sensitive Method to Evaluate Gap Junctional Coupling / |r Luc Leybaert and Michael J. Sanderson -- |g 24. |t Biochemical Analysis of Connexin Phosphorylation / |r Bonnie J. Warn-Cramer, Wendy E. Kurata and Alan F. Lau -- |g 25. |t How to Close a Gap Junction Channel: Efficacies and Potencies of Uncoupling Agents / |r Renato Rozental, Miduturu Srinivas and David C. Spray. |
| 650 | 0 | |a Connexins |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Connexins |x physiology |v Laboratory Manuals. |
| 650 | 2 | 2 | |a Gap Junctions |x physiology |v Laboratory Manuals. |
| 650 | 7 | |a Connexins. |2 fast |0 http://id.worldcat.org/fast/fst00875361 | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 http://id.worldcat.org/fast/fst01920776 | |
| 700 | 1 | |a Bruzzone, Roberto. |0 http://id.loc.gov/authorities/names/n00000856 |1 http://viaf.org/viaf/46944030 | |
| 700 | 1 | |a Giaume, Christian. |0 http://id.loc.gov/authorities/names/n00000857 |1 http://viaf.org/viaf/71445301 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 154. | |
| 901 | |a ToCBNA |a Analytic | ||
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| 927 | |t Library of Congress classification |a QH506.M45 v.154 |l ASR |c ASR-SciASR |g Analytic |e CRERAR |b A56635999 |i 7962883 | ||
