Connexin methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2001. |
|---|---|
| Description: | xiv, 491 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v.154 Methods in molecular biology (Clifton, N.J.) ; v. 154. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845240 |
Table of Contents:
- Preface
- Contributors
- Part I. Tools to Study Connexins
- 1. Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques
- 2. Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions
- 3. Purification of Gap Junctions
- 4. Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions
- 5. Connexins/Connexons: Cell-Free Expression
- 6. Biochemical Analysis of Connexon Assembly
- 7. Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells
- 8. Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction
- 9. Use of Retroviruses to Express Connexins
- 10. Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides
- 11. Transfection and Expression of Exogenous Connexins in Mammalian Cells
- Part II. Assays for Function
- 12. Assaying the Molecular Permeability of Connexin Channels
- 13. Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins
- 14. Mutagenesis to Study Channel Structure
- 15. Dual Patch Clamp
- 16. Determining Ionic Permeabilities of Gap Junction Channels
- 17. Fluorescence Recovery After Photobleaching
- 18. Capture of Transjunctional Metabolites
- Part III. Physiology and Biology of Connexins
- 19. The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes
- 20. Exploring Hemichannel Permeability In Vitro
- 21. Inducing De Novo Formation of Gap Juntion Channels
- 22. Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain
- 23. Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds: A Sensitive Method to Evaluate Gap Junctional Coupling
- 24. Biochemical Analysis of Connexin Phosphorylation
- 25. How to Close a Gap Junction Channel: Efficacies and Potencies of Uncoupling Agents
- Index
