DNA sequencing protocols /
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Edition: | 2nd ed. |
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Imprint: | Totowa, NJ : Humana Press, c2001. |
Description: | x, 244 p. : ill. (some col.) ; 24 cm. |
Language: | English |
Series: | Methods in molecular biology ; 167 Methods in molecular biology (Clifton, N.J.) ; v. 167. |
Subject: | |
Format: | Print Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845245 |
Table of Contents:
- Preface
- Contributors
- 1. Introduction to DNA Sequencing
- 2. The Universal Primers and the Shotgun DNA Sequencing Method
- 3. M13 Sequencing
- 4. Primer Design and Primer-Directed Sequencing
- 5. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction
- 6. Solid Phase Fluorescent Sequencing of the CFTR Gene
- 7. Shotgun DNA Sequencing
- 8. Cycle Sequencing
- 9. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers
- 10. Automated Fluorescent DNA Sequencing on the ABI PRISM 377
- 11. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer
- 12. Fluorescent Sequencing Protocols for the ALF
- 13. Fluorescent Sequencing for Heterozygote Mutation Detection
- 14. Sequence Databases and the Internet
- 15. DNA Sequencing by Capillary Array Electrophoresis
- Index