DNA sequencing protocols /

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Bibliographic Details
Edition:2nd ed.
Imprint:Totowa, NJ : Humana Press, c2001.
Description:x, 244 p. : ill. (some col.) ; 24 cm.
Language:English
Series:Methods in molecular biology ; 167
Methods in molecular biology (Clifton, N.J.) ; v. 167.
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/5845245
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Other authors / contributors:Graham, Colin A.
Hill, Alison J. M.
ISBN:0896037169 (alk. paper)
0896037215 (pbk.)
Notes:Includes bibliographical references and index.
Table of Contents:
  • Preface
  • Contributors
  • 1. Introduction to DNA Sequencing
  • 2. The Universal Primers and the Shotgun DNA Sequencing Method
  • 3. M13 Sequencing
  • 4. Primer Design and Primer-Directed Sequencing
  • 5. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction
  • 6. Solid Phase Fluorescent Sequencing of the CFTR Gene
  • 7. Shotgun DNA Sequencing
  • 8. Cycle Sequencing
  • 9. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers
  • 10. Automated Fluorescent DNA Sequencing on the ABI PRISM 377
  • 11. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer
  • 12. Fluorescent Sequencing Protocols for the ALF
  • 13. Fluorescent Sequencing for Heterozygote Mutation Detection
  • 14. Sequence Databases and the Internet
  • 15. DNA Sequencing by Capillary Array Electrophoresis
  • Index