Gene knockout protocols /

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Bibliographic Details
Imprint:	Totowa, NJ : Humana Press, c2001.
Description:	xi, 431 p. : ill. ; 24 cm.
Language:	English
Series:	Methods in molecular biology ; v. 158 Methods in molecular biology (Clifton, N.J.) ; v. 158.
Subject:	Gene targeting -- Laboratory manuals. Transgenic mice -- Laboratory manuals. Gene Targeting -- methods -- Laboratory Manuals. Mice, Knockout -- Laboratory Manuals. Mice, Transgenic -- Laboratory Manuals. Gene targeting. Transgenic mice. Laboratory manuals.
Format:	Print Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/5845251

Hidden Bibliographic Details
Other authors / contributors:	Tymms, Martin J. Kola, Ismail.
ISBN:	0896035727 (alk. paper)
Notes:	Includes bibliographical references and index.

Table of Contents:

Preface
Contributors
1. Overview: Gene Knockouts
2. Isolation and Maintenance of Primate Embryonic Stem Cells
3. Gene Targeting in ES Cells
4. Manipulating Mouse Embryonic Stem Cells
5. Gene Targeting in a Centralized Facility
6. The LoxP/CRE System and Genome Modification
7. Creation and Use of a Cre Recombinase Transgenic Database
8. Choice of Mouse Strains for Gene Targeting
9. Isolation, Microinjection, and Transfer of Mouse Blastocysts
10. Aggregation Chimeras: Combining ES Cells, Diploid and Tetraploid Embryos
11. How to Study Pathologic Phenotypes of Knockout Mice
12. Analysis of Hematopoietic Phenotypes in Knockout Mouse Models
13. Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function
14. Influence of Genetic Background on Knockout Mouse Phenotypes
15. Lineage Allocation During Early Embryogenesis: Mapping of the Neural Primordia and Application to the Analysis of Mouse Mutants
16. Generation of Double-Knockout Embryonic Stem Cells
17. In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes
18. Embryonic Stem Cells in the Study of Hematopoiesis
19. Interferon-Inducible ES Cell Expression Systems
20. Transgenic Studies in the Mouse: Improving the Technology Towards a Conditional Temporal and Spatial Approach
21. In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping
22. Epigenetic Effects on Transgene Expression
23. Positional-Candidate Cloning of Genes from Mouse Mutants
24. Genetically Engineered Mice: Husbandry and Resources
25. Embryo Cryopreservation for Transgenic Mouse Lines
Index

Gene knockout protocols /

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