Matrix metalloproteinase protocols /
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Imprint: | Totowa, N.J. : Humana Press, c2001. |
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Description: | xv, 545 p. : ill. (some col.) ; 24 cm. |
Language: | English |
Series: | Methods in molecular biology ; 151 Methods in molecular biology (Clifton, N.J.) ; v. 151. |
Subject: | |
Format: | Print Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845261 |
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245 | 0 | 0 | |a Matrix metalloproteinase protocols / |c edited by Ian M. Clark. |
260 | |a Totowa, N.J. : |b Humana Press, |c c2001. | ||
300 | |a xv, 545 p. : |b ill. (some col.) ; |c 24 cm. | ||
336 | |a text |b txt |2 rdacontent |0 http://id.loc.gov/vocabulary/contentTypes/txt | ||
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338 | |a volume |b nc |2 rdacarrier |0 http://id.loc.gov/vocabulary/carriers/nc | ||
490 | 1 | |a Methods in molecular biology ; |v 151 | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | 0 | |g Part I.. |t MMPs and TIMPs--An Overview of the Field -- |g 1. |t MMPs and TIMPs: An Historical Perspective / |r J. Frederick Woessner, Jr. -- |g 2. |t Strategies for Cloning New MMPs and TIMPs / |r Gloria Velasco and Carlos Lopez-Otin -- |g 3. |t Structural Studies on MMPs and TIMPs / |r Wolfram Bode and Klaus Maskos -- |g 4. |t Matrix Metalloproteinase Substrate Binding Domains, Modules and Exosites: Overview and Experimental Strategies / |r Christopher M. Overall -- |g 5. |t The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcriptional and Posttranscriptional Regulation, Signal Transduction and Cell-Type-Specific Expression / |r Matthew P. Vincenti -- |g 6. |t Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice / |r Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway and Zena Werb -- |g Part II.. |t Expression and Purification of MMPs and TIMPs -- |g 7. |t Expression of MMPs and TIMPs in Mammalian Cells / |r Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy and Dylan R. Edwards -- |g 8. |t Expression of Recombinant Matrix Metalloproteinases in Escherichia coli / |r L. Jack Windsor and Darin L. Steele -- |g 9. |t Expression of Human Collagenase I (MMP-1) and TIMP-1 in a Baculovirus-Based Expression System / |r Rudiger Vallon and Peter Angel -- |g 10. |t Expression of Recombinant Matrix Metalloproteinases in Yeast / |r Glenn A. Doyle -- |g 11. |t Expression of Recombinant Membrane-Type MMPs / |r Michael J. Butler, Marie-Pia d'Ortho and Susan J. Atkinson -- |g 12. |t Refolding of TIMP-2 from Escherichia coli Inclusion Bodies / |r Richard A. Williamson -- |g 13. |t Expression and Refolding of Full-Length Human TIMP-1 / |r Deborah Davis -- |g 14. |t Purification of MMPs and TIMPs / |r Ken-ichi Shimokawa and Hideaki Nagase -- |g Part III.. |t Detection of MMPs and TIMPs -- |g 15. |t Monitoring MMP and TIMP mRNA Expression by RT-PCR / |r Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth, Gilbert A. Schultz, Steven J. Wall and Dylan R. Edwards -- |g 16. |t Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA / |r Anne M. Delany -- |g 17. |t In Situ Hybridization for Metalloproteinases and Their Inhibitors / |r Tina L. Hurskainen and Suneel S. Apte -- |g 18. |t Use of EIA to Measure MMPs and TIMPs / |r Noboru Fujimoto and Kazushi Iwata -- |g 19. |t Immunohistochemistry of MMPs and TIMPs / |r Yasunori Okada -- |g 20. |t Detecting Polymorphisms in MMP Genes / |r Shu Ye and Adriano M. Henney -- |g Part IV.. |t Assay of MMP and TIMP Activities -- |g 21. |t Methods for Studying Activation of Matrix Metalloproteinases / |r Vera Knauper and Gillian Murphy -- |g 22. |t Assay of Matrix Metalloproteinases Against Matrix Substrates / |r Timothy E. Cawston, Paul Koshy and Andrew D. Rowan -- |g 23. |t Zymography and Reverse Zymography for Detecting MMPs and TIMPs / |r Susan P. Hawkes, Hongxia Li and Gary T. Taniguchi -- |g 24. |t In Situ Zymography / |r Sarah J. George and Jason L. Johnson -- |g 25. |t Detection of Focal Proteolysis Using Texas-Red-Gelatin / |r Rosalind M. Hembry -- |g 26. |t Antibodies to MMP-Cleaved Aggrecan / |r Amanda J. Fosang, Karena Last, David C. Jackson and Lorena Brown -- |g 27. |t Cartilage Proteoglycan Release Assay / |r Caron J. Billington -- |g 28. |t Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens / |r R. Clark Billinghurst, Mirela Ionescu and A. Robin Poole -- |g 29. |t Collagen Degradation Assays / |r Anthony P. Hollander -- |g 30. |t Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium Iodide Labeling and Confocal Laser Scanning Microscopy / |r Ulrike Benbow, Kenneth A. Orndorff and Alice L. Givan -- |g 31. |t Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases / |r Gregg B. Fields -- |g 32. |t Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP) / |r Mike Hutton and Frances Willenbrock -- |g 33. |t Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases / |r Kyoko Yamashita and Taro Hayakawa. |
650 | 0 | |a Metalloproteinases |v Laboratory manuals. | |
650 | 0 | |a Extracellular matrix proteins |v Laboratory manuals. | |
650 | 1 | 2 | |a Matrix Metalloproteinases |v Laboratory Manuals. |
650 | 7 | |a Extracellular matrix proteins. |2 fast |0 http://id.worldcat.org/fast/fst00918997 | |
650 | 7 | |a Metalloproteinases. |2 fast |0 http://id.worldcat.org/fast/fst01017929 | |
655 | 7 | |a Laboratory manuals. |2 fast |0 http://id.worldcat.org/fast/fst01920776 | |
700 | 1 | |a Clark, Ian M. |0 http://id.loc.gov/authorities/names/n99253129 |1 http://viaf.org/viaf/14104935 | |
830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 151. | |
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