Matrix metalloproteinase protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, c2001.
Description:xv, 545 p. : ill. (some col.) ; 24 cm.
Language:English
Series:Methods in molecular biology ; 151
Methods in molecular biology (Clifton, N.J.) ; v. 151.
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/5845261
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Other authors / contributors:Clark, Ian M.
ISBN:0896037339 (alk. paper)
Notes:Includes bibliographical references and index.

MARC

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300 |a xv, 545 p. :  |b ill. (some col.) ;  |c 24 cm. 
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490 1 |a Methods in molecular biology ;  |v 151 
504 |a Includes bibliographical references and index. 
505 0 0 |g Part I..  |t MMPs and TIMPs--An Overview of the Field --  |g 1.  |t MMPs and TIMPs: An Historical Perspective /  |r J. Frederick Woessner, Jr. --  |g 2.  |t Strategies for Cloning New MMPs and TIMPs /  |r Gloria Velasco and Carlos Lopez-Otin --  |g 3.  |t Structural Studies on MMPs and TIMPs /  |r Wolfram Bode and Klaus Maskos --  |g 4.  |t Matrix Metalloproteinase Substrate Binding Domains, Modules and Exosites: Overview and Experimental Strategies /  |r Christopher M. Overall --  |g 5.  |t The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcriptional and Posttranscriptional Regulation, Signal Transduction and Cell-Type-Specific Expression /  |r Matthew P. Vincenti --  |g 6.  |t Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice /  |r Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway and Zena Werb --  |g Part II..  |t Expression and Purification of MMPs and TIMPs --  |g 7.  |t Expression of MMPs and TIMPs in Mammalian Cells /  |r Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy and Dylan R. Edwards --  |g 8.  |t Expression of Recombinant Matrix Metalloproteinases in Escherichia coli /  |r L. Jack Windsor and Darin L. Steele --  |g 9.  |t Expression of Human Collagenase I (MMP-1) and TIMP-1 in a Baculovirus-Based Expression System /  |r Rudiger Vallon and Peter Angel --  |g 10.  |t Expression of Recombinant Matrix Metalloproteinases in Yeast /  |r Glenn A. Doyle --  |g 11.  |t Expression of Recombinant Membrane-Type MMPs /  |r Michael J. Butler, Marie-Pia d'Ortho and Susan J. Atkinson --  |g 12.  |t Refolding of TIMP-2 from Escherichia coli Inclusion Bodies /  |r Richard A. Williamson --  |g 13.  |t Expression and Refolding of Full-Length Human TIMP-1 /  |r Deborah Davis --  |g 14.  |t Purification of MMPs and TIMPs /  |r Ken-ichi Shimokawa and Hideaki Nagase --  |g Part III..  |t Detection of MMPs and TIMPs --  |g 15.  |t Monitoring MMP and TIMP mRNA Expression by RT-PCR /  |r Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth, Gilbert A. Schultz, Steven J. Wall and Dylan R. Edwards --  |g 16.  |t Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA /  |r Anne M. Delany --  |g 17.  |t In Situ Hybridization for Metalloproteinases and Their Inhibitors /  |r Tina L. Hurskainen and Suneel S. Apte --  |g 18.  |t Use of EIA to Measure MMPs and TIMPs /  |r Noboru Fujimoto and Kazushi Iwata --  |g 19.  |t Immunohistochemistry of MMPs and TIMPs /  |r Yasunori Okada --  |g 20.  |t Detecting Polymorphisms in MMP Genes /  |r Shu Ye and Adriano M. Henney --  |g Part IV..  |t Assay of MMP and TIMP Activities --  |g 21.  |t Methods for Studying Activation of Matrix Metalloproteinases /  |r Vera Knauper and Gillian Murphy --  |g 22.  |t Assay of Matrix Metalloproteinases Against Matrix Substrates /  |r Timothy E. Cawston, Paul Koshy and Andrew D. Rowan --  |g 23.  |t Zymography and Reverse Zymography for Detecting MMPs and TIMPs /  |r Susan P. Hawkes, Hongxia Li and Gary T. Taniguchi --  |g 24.  |t In Situ Zymography /  |r Sarah J. George and Jason L. Johnson --  |g 25.  |t Detection of Focal Proteolysis Using Texas-Red-Gelatin /  |r Rosalind M. Hembry --  |g 26.  |t Antibodies to MMP-Cleaved Aggrecan /  |r Amanda J. Fosang, Karena Last, David C. Jackson and Lorena Brown --  |g 27.  |t Cartilage Proteoglycan Release Assay /  |r Caron J. Billington --  |g 28.  |t Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens /  |r R. Clark Billinghurst, Mirela Ionescu and A. Robin Poole --  |g 29.  |t Collagen Degradation Assays /  |r Anthony P. Hollander --  |g 30.  |t Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium Iodide Labeling and Confocal Laser Scanning Microscopy /  |r Ulrike Benbow, Kenneth A. Orndorff and Alice L. Givan --  |g 31.  |t Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases /  |r Gregg B. Fields --  |g 32.  |t Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP) /  |r Mike Hutton and Frances Willenbrock --  |g 33.  |t Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases /  |r Kyoko Yamashita and Taro Hayakawa. 
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