Matrix metalloproteinase protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2001. |
|---|---|
| Description: | xv, 545 p. : ill. (some col.) ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; 151 Methods in molecular biology (Clifton, N.J.) ; v. 151. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845261 |
Table of Contents:
- Preface
- Contributors
- Part I.. MMPs and TIMPs--An Overview of the Field
- 1. MMPs and TIMPs: An Historical Perspective
- 2. Strategies for Cloning New MMPs and TIMPs
- 3. Structural Studies on MMPs and TIMPs
- 4. Matrix Metalloproteinase Substrate Binding Domains, Modules and Exosites: Overview and Experimental Strategies
- 5. The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcriptional and Posttranscriptional Regulation, Signal Transduction and Cell-Type-Specific Expression
- 6. Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice
- Part II.. Expression and Purification of MMPs and TIMPs
- 7. Expression of MMPs and TIMPs in Mammalian Cells
- 8. Expression of Recombinant Matrix Metalloproteinases in Escherichia coli
- 9. Expression of Human Collagenase I (MMP-1) and TIMP-1 in a Baculovirus-Based Expression System
- 10. Expression of Recombinant Matrix Metalloproteinases in Yeast
- 11. Expression of Recombinant Membrane-Type MMPs
- 12. Refolding of TIMP-2 from Escherichia coli Inclusion Bodies
- 13. Expression and Refolding of Full-Length Human TIMP-1
- 14. Purification of MMPs and TIMPs
- Part III.. Detection of MMPs and TIMPs
- 15. Monitoring MMP and TIMP mRNA Expression by RT-PCR
- 16. Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA
- 17. In Situ Hybridization for Metalloproteinases and Their Inhibitors
- 18. Use of EIA to Measure MMPs and TIMPs
- 19. Immunohistochemistry of MMPs and TIMPs
- 20. Detecting Polymorphisms in MMP Genes
- Part IV.. Assay of MMP and TIMP Activities
- 21. Methods for Studying Activation of Matrix Metalloproteinases
- 22. Assay of Matrix Metalloproteinases Against Matrix Substrates
- 23. Zymography and Reverse Zymography for Detecting MMPs and TIMPs
- 24. In Situ Zymography
- 25. Detection of Focal Proteolysis Using Texas-Red-Gelatin
- 26. Antibodies to MMP-Cleaved Aggrecan
- 27. Cartilage Proteoglycan Release Assay
- 28. Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens
- 29. Collagen Degradation Assays
- 30. Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium Iodide Labeling and Confocal Laser Scanning Microscopy
- 31. Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
- 32. Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP)
- 33. Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases
- Index
