Nuclease methods and protocols /
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| Imprint: | Totowa, NJ : Humana Press, c2001. |
|---|---|
| Description: | xvii, 525 p. : ill. (some col.) ; 26 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 160 Methods in molecular biology (Clifton, N.J.) ; v. 160. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845264 |
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| 245 | 0 | 0 | |a Nuclease methods and protocols / |c edited by Catherine H. Schein. |
| 260 | |a Totowa, NJ : |b Humana Press, |c c2001. | ||
| 300 | |a xvii, 525 p. : |b ill. (some col.) ; |c 26 cm. | ||
| 336 | |a text |b txt |2 rdacontent |0 http://id.loc.gov/vocabulary/contentTypes/txt | ||
| 337 | |a unmediated |b n |2 rdamedia |0 http://id.loc.gov/vocabulary/mediaTypes/n | ||
| 338 | |a volume |b nc |2 rdacarrier |0 http://id.loc.gov/vocabulary/carriers/nc | ||
| 490 | 1 | |a Methods in molecular biology ; |v v. 160 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |g Part I.. |t Characterizing Nuclease Activity -- |g 1. |t Processivity of DNA Repair Enzymes / |r R. Stephen Lloyd -- |g 2. |t Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribonucleases / |r M. Victoria Nogues and Claudi M. Cuchillo -- |g 3. |t Ire1p: A Kinase and Site-Specific Endoribonuclease / |r Tania N. Gonzalez and Peter Walter -- |g 4. |t Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases / |r Gregor Meiss, Oleg Gimadutdinow, Peter Friedhoff and Alfred M. Pingoud -- |g 5. |t Quantitating mRNAs with Relative and Competitive RT-PCR / |r Ellen A. Prediger -- |g 6. |t Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants / |r Brian Beecher and Bruce A. McClure -- |g 7. |t Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family / |r Francesco Acquati, Cinzia Nucci, Marco G. Bianchi, Tatiana Gorletta and Roberto Taramelli -- |g Part II.. |t Inhibitors and Activators of Nucleases -- |g 8. |t Ribonuclease Inhibitors / |r Brittan L. Pasloske -- |g 9. |t Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon-[gamma] In Vitro / |r Catherine H. Schein -- |g 10. |t Assays for the Evaluation of HIV-1 Integrase Inhibitors / |r Zeger Debyser, Peter Cherepanov, Wim Pluymers and Erik De Clercq -- |g 11. |t The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides / |r Richard V. Giles and David M. Tidd -- |g 12. |t The 2-5A/RNase L Pathway and Inhibition by RNase L[middle dot]Inhibitor (RLI) / |r Catherine Bisbal, Tamim Salehzada, Michelle Silhol, Camille Martinand, Florence Le Roy and Bernard Lebleu -- |g Part III.. |t Relating Nuclease Structure and Function -- |g 13. |t Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein Complex / |r Bostjan Kobe -- |g 14. |t Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar / |r Gideon Schreiber -- |g 15. |t Assaying In Vitro Refolding of RNases by Mass Spectrometry / |r Gennaro Marino, Piero Pucci and Margherita Ruoppolo -- |g 16. |t Dissecting Nucleases into Their Structural and Functional Domains: Mapping the RNA-Binding Surface of RNase III by NMR / |r Andres Ramos and Annalisa Pastore -- |g 17. |t Using Electrostatics to Define the Active Site of Serratia Endonuclease / |r Kurt L. Krause and Mitchell D. Miller -- |g 18. |t Homology Modeling and Simulations of Nuclease Structures / |r Kizhake V. Soman, Catherine H. Schein, Hongyao Zhu and Werner Braun -- |g 19. |t Methods for Determining Activity and Specificity of DNA Binding and DNA Cleavage by Class II Restriction Endonucleases / |r Albert Jeltsch and Alfred M. Pingoud -- |g 20. |t Engineered Properties and Assays for Human DNase I Mutants / |r Clark Q. Pan, Dominick V. Sinicropi and Robert A. Lazarus -- |g Part IV.. |t Nucleases in the Clinic -- |g 21. |t Assays for Human DNase I Activity in Biological Matrices / |r Dominick V. Sinicropi and Robert A. Lazarus -- |g 22. |t Evaluation of Retroviral Ribonuclease H Activity / |r Jennifer T. Miller, Jason W. Rausch and Stuart F. J. Le Grice -- |g 23. |t Assays for Detection of RNase A Superfamily Ribonucleases / |r Helene F. Rosenberg and Joseph B. Domachowske -- |g 24. |t Assay for Antitumor and Lectin Activity in RNase Homologs / |r Kazuo Nitta -- |g 25. |t Isolation and Enzymatic Activity of Angiogenin / |r James F. Riordan and Robert Shapiro -- |g 26. |t Preparation and Preclinical Characterization of RNase-Based Immunofusion Proteins / |r Dianne L. Newton and Susanna M. Rybak -- |g Part V.. |t Assays Using Nucleases -- |g 27. |t Restriction Endonucleases and Their Uses / |r Raymond J. Williams -- |g 28. |t Isolation and Characterization of an Unknown Restriction Endonuclease / |r Raymond J. Williams -- |g 29. |t Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way / |r Shelby L. Berger -- |g 30. |t Techniques to Measure Nucleic Acid-Protein Binding and Specificity: Nuclear Extract Preparations, DNase I Footprinting, and Mobility Shift Assays / |r Richard A. Rippe, David A. Brenner and Antonio Tugores -- |g 31. |t Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping / |r Jan Kormanec -- |g 32. |t Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays / |r Ellen A. Prediger. |
| 650 | 0 | |a Nucleases |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Deoxyribonucleases |v Laboratory Manuals. |
| 650 | 1 | 2 | |a Ribonucleases |v Laboratory Manuals. |
| 650 | 2 | 2 | |a Endonucleases |v Laboratory Manuals. |
| 650 | 7 | |a Nucleases. |2 fast |0 http://id.worldcat.org/fast/fst01041058 | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 http://id.worldcat.org/fast/fst01920776 | |
| 700 | 1 | |a Schein, Catherine H. |0 http://id.loc.gov/authorities/names/n00000242 |1 http://viaf.org/viaf/38142501 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 160. | |
| 901 | |a ToCBNA |a Analytic | ||
| 903 | |a HeVa | ||
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| 928 | |t Library of Congress classification |a QH506.M45 v.160 |l ASR |c ASR-SciASR |i 4993490 | ||
| 927 | |t Library of Congress classification |a QH506.M45 v.160 |l ASR |c ASR-SciASR |g Analytic |e CRERAR |b A57250207 |i 7962906 | ||
