Protein kinase protocols /
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| Imprint: | Totowa, N.J. : Humana Press, 2001. |
|---|---|
| Description: | xi, 365 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v.124 Methods in molecular biology (Clifton, N.J.) ; v. 124. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845266 |
Table of Contents:
- Preface
- Contributors
- 1. Protein Kinase-Mediated Signaling Networks: Regulation and Functional Characterization
- 2. Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies
- 3. Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins
- 4. Two-Dimensional Phosphoamino Acid Analysis
- 5. Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases
- 6. Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry
- 7. Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases
- 8. Assays for Mitogen-Activated Protein Kinase (MAPK) Subtypes and MAPK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate
- 9. JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins: Analysis by Phosphotyrosine Blotting and EMSA
- 10. Assays for Glycogen Synthase Kinase-3 (GSK-3)
- 11. Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements
- 12. Protein Histidine Kinase
- 13. Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System
- 14. Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins
- 15. Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay
- 16. Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries
- 17. Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of Receptor Targets (CORT) Strategy
- 18. Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System
- 19. CDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System
- 20. Analysis of SH2 Domain--Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance
- 21. Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins
- 22. Isolation and Characterization of "Orphan-RTK" Ligands Using an Integrated Biosensor Approach
- Index
