Antibody phage display : methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2002. |
|---|---|
| Description: | xiii, 401 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 178 Methods in molecular biology (Clifton, N.J.) ; v. 178. |
| Subject: | |
| Format: | E-Resource Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845278 |
Table of Contents:
- Preface
- Contributors
- 1. Overview of Antibody Phage-Display Technology and Its Applications
- 2. Standard Protocols for the Construction of Fab Libraries
- 3. Standard Protocols for the Construction of scFv Libraries
- 4. Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice
- 5. Construction of Large Naive Fab Libraries
- 6. Construction of Polyclonal Antibody Libraries Using Phage Display
- 7. Antigen-Driven Stimulation of B-Lymphocytes In Vitro
- 8. The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion
- 9. Panning of Antibody Phage-Display Libraries: Standard Protocols
- 10. Selection of Antibodies Against Biotinylated Antigens
- 11. Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries
- 12. Blocking Immunodominant Epitopes by Competitive Deselection
- 13. Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy
- 14. Screening of Phage-Expressed Antibody Libraries by Capture Lift
- 15. Antibody-Guided Selection Using Capture-Sandwich ELISA
- 16. Proximity-Guided (ProxiMol) Antibody Selection
- 17. Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
- 18. Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques
- 19. Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs
- 20. Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments
- 21. Selection of Antivodi's Based on Antibody Kinetic Binding Properties
- 22. Selection of Functional Antibodies on the Basis of Valency
- 23. Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis
- 24. Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement
- 25. Error-Prone Polymerase Chain Reaction for Modification of scFvs
- 26. Use of Escherichia coli Mutator Cells to Mature Antibodies
- 27. Chain Shuffling to Modify Properties of Recombinant Immunoglobulins
- 28. Generation of Bispecific and Tandem Diabodies
- 29. High-Level Periplasmic Expression and Purification of scFvs
- 30. Periplasmic Expression and Purification of Recombinant Fabs
- 31. Expression of Antibody Fragments in Pichia pastoris
- 32. Expression of V HH Antibody Fragments in Saccharomyces cerevisiae
- 33. Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments
- 34. Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
- 35. Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells
- Index
