Embryonic stem cells : methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2002. |
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| Description: | xvi, 499 p. : ill. (some col.) ; cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 185 Methods in molecular biology (Clifton, N.J.) ; v. 185. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845286 |
| Summary: | Embryonic stem cells are one of the most discussed biomedical issues of the day as they have generated new possibilities for increasing our understanding of cell lineages and differentiation. ES cells are providing a limitless source of specific cell populations, and this book provides the detailed methods for use of ES cells to study various lineages and tissue types. The first part of the volume includes detailed protocols for ES cell isolation, maintenance, modulation of gene expression, and studies of ES cell cycle and apoptosis. It also provides the necessary protocols to generate diverse cell and tissue types including blood, endothelium, skeletal muscle, cardiac muscle, neurons, osteoclasts, melonocytes, and keratinocytes. |
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| Physical Description: | xvi, 499 p. : ill. (some col.) ; cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 0896038815 |
