Embryonic stem cells : methods and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2002. |
|---|---|
| Description: | xvi, 499 p. : ill. (some col.) ; cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 185 Methods in molecular biology (Clifton, N.J.) ; v. 185. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845286 |
Table of Contents:
- Preface
- Contributors
- Color Plates
- 1. Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
- 2. The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos
- 3. Analysis of the Cell Cycle in Mouse Embryonic Stem Cells Pierre Savatier
- 4. Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
- 5. Effects of Altered Gene Expression on ES Cell Differentiation
- 6. Hypoxic Gene Regulation in Differentiating ES Cells
- 7. Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells
- 8. Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development
- 9. Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System
- 10. Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development Toshiyuki Yamane
- 11. Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells
- 12. Embryonic Stem Cell Differentiation and the Vascular Lineage
- 13. Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
- 14. Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
- 15. Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
- 16. Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells
- 17. Lineage Selection for Generation and Amplification of Neural Precursor Cells
- 18. Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson's Disease
- 19. Epidermal Lineage
- 20. ES Cell Differentiation Into the Hair Follicle Lineage In Vitro
- 21. Embryonic Stem Cells as a Model for Studying Melanocyte Development
- 22. Using Progenitor Cells and Gene Chips to Define Genetic Pathways
- 23. ES Cell-Mediated Conditional Transgenesis
- 24. Switching on Lineage Tracers Using Site-Specific Recombination
- 25. From ES Cells to Mice: The Gene Trap Approach
- 26. Functional Genomics by Gene-Trapping in Embryonic Stem Cells
- 27. Phage-Displayed Antibodies to Detect Cell Markers
- 28. Gene Transfer Using Targeted Filamentous Bacteriophage
- 29. Single-Cell PCR Methods for Studying Stem Cells and Progenitors
- 30. Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays
- 31. Expression Profiling Using Quantitative Hybridization on Macroarrays
- 32. Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells
- 33. Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
- 34. Direct Selection of cDNAs by Phage Display
- 35. Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells
- Index
