Oxidative stress biomarkers and antioxidant protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2002. |
|---|---|
| Description: | xiv, 322 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 186 Methods in molecular biology (Clifton, N.J.) ; v. 186. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845296 |
Table of Contents:
- Preface
- Contributors
- Part I. Techniques for Free Radical Derived Biomarkers
- 1. Human Xanthine Oxidoreductase Determination by a Competitive ELISA
- 2. Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC
- 3. Determination of Products of Lipid Oxidation by Infrared Spectroscopy
- 4. Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry
- 5. Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies
- 6. Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes
- 7. Mass Spectrometric Quantification of F 2 -Isoprostanes as Indicators of Oxidative Stress
- 8. Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein
- 9. Detection of Certain Peroxynitrite-Induced DNA Modifications
- 10. Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS
- 11. Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins
- 12. Rapid Determination of Glutamate Using HPLC Technology
- 13. A Rapid Method for the Quantification of GSH and GSSG in Biological Samples
- 14. Protein Carbonyl Measurement by ELISA
- 15. N ϵ -(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in Long-Lived Tissue Proteins
- 16. Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis
- 17. Oxidation of Cellular DNA Measured with the Comet Assay
- 18. Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis
- 19. Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA
- Part II. Techniques for Antioxidant Biomarkers
- 20. Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices
- 21. Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode
- 22. Measurement of a-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry
- 23. Analysis of Tocotrienols in Different Sample Matrixes by HPLC
- 24. Measurement of ß-Carotene15,15'-Dioxygenase Activity by Reverse-Phase HPLC
- 25. Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress
- 26. Catechol- and Pyrogallol-Type Flavonoids: Analysis of Tea Catechins in Plasma
- 27. Pyruvate Dehydrogenase Complex as a Marker of Mitochondrial Metabolism: Inhibition by 4-Hydroxy-2-Nonenal
- 28. Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and In Situ Oxidase Activity
- 29. Metallothionein Determination by Isocratic HPLC with Fluorescence Derivatization
- 30. Quantification of Oxidized Metallothionein by a Cd-Saturation Method
- 31. Fractionation of Herbal Medicine for Identifying Antioxidant Activity
- 32. Designing Safer (Soft) Drugs by Avoiding the Formation of Toxic and Oxidative Metabolites
- 33. Statistical Correction of the Area Under the ROC Curve in the Presence of Random Measurement Error and Applications to Biomarkers of Oxidative Stress
- Index
