PCR cloning protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, c2002. |
| Description: | xiv, 439 p. : ill. ; 23 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; 192 Methods in molecular biology (Clifton, N.J.) ; v. 192. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845297 |
Table of Contents:
- Preface
- Contributors
- Part I.. Performing and Optimizing PCR
- 1. Polymerase Chain Reaction: Basic Principles and Routine Practice
- 2. Computer Programs for PCR Primer Design and Analysis
- 3. Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming
- 4. XL PCR Amplification of Long Targets from Genomic DNA
- 5. Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments
- 6. Long Distance Reverse-Transcription PCR
- 7. Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues
- 8. GC-Rich Template Amplification by Inverse PCR: DNA Polymerase and Solvent Effects
- 9. PCR Procedure for the Isolation of Trinucleotide Repeats
- 10. Methylation-Specific PCR
- 11. Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads
- Part II.. Cloning PCR Products
- 12. Cloning PCR Products: An Overview
- 13. Using T4 DNA Polymerase to Generate Clonable PCR Products
- 14. Enzyme-Free Cloning of PCR Products and Fusion Protein Expression
- 15. Directional Restriction Site-Free Insertion of PCR Products into Vectors
- 16. Autosticky PCR: Directional Cloning of PCR Products with Preformed 5' Overhangs
- 17. A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses
- Part III.. Mutagenesis and Recombination
- 18. PCR Approaches to DNA Mutagenesis and Recombination: An Overview
- 19. In-Frame Cloning of Synthetic Genes Using PCR Inserts
- 20. Megaprimer PCR
- 21. PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones
- 22. PCR Method for Generating Multiple Mutations at Adjacent Sites
- 23. A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes
- 24. PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
- 25. Staggered Extension Process (StEP) In Vitro Recombination
- 26. Random Mutagenesis by Whole-Plasmid PCR Amplification
- Part IV.. Cloning Unknown Neighboring DNA
- 27. PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants: An Overview
- 28. Long Distance Vectorette PCR (LDV PCR)
- 29. Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ("Cold-Start Method")
- 30. Inverse PCR: cDNA Cloning
- 31. Inverse PCR: Genomic DNA Cloning
- 32. Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers
- 33. The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR
- 34. Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis
- 35. Isolation of Large Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR
- 36. A "Step Down" PCR-Based Technique for Walking Into and the Subsequent Direct Sequence Analysis of Flanking Genomic DNA
- Part V.. Library Construction and Screening
- 37. Use of PCR in Library Screening: An Overview
- 38. Cloning of Homologous Genes by Gene-Capture PCR
- 39. Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
- 40. Rapid cDNA Cloning by PCR Screening (RC-PCR)
- 41. Generation and PCR Screening of Bacteriophage [lambda] Sublibraries Enriched for Rare Clones (the "Sublibrary Method")
- 42. PCR-Based Screening for Bacterial Artificial Chromosome Libraries
- 43. A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method
- 44. A Microtiter-Plate-Based High Throughput PCR Product Purification Method
- Index
