Integrin protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, 1999.
Description:xi, 296 p. : ill. ; 24 cm.
Language:English
Series:Methods in molecular biology ; v. 129
Methods in molecular biology (Clifton, N.J.) ; v. 129.
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/5845309
Hidden Bibliographic Details
Other authors / contributors:Howlett, Anthony.
ISBN:0896035697 (alk. paper)
Notes:Includes bibliographical references and index.

MARC

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490 1 |a Methods in molecular biology ;  |v v. 129 
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505 0 |a pt. 1. Working with integrins -- Integrin-binding peptides derived from phage display libraries -- Production of rat monoclonal antibiodies specific for mouse integrins -- Biochemical analysis of integrin-mediated Shc signaling -- Assays for kinase activity -- Immunoblotting of integrins -- Application of flow cytometry in the analysis and sterile sorting of cell populations based on integrin expression -- Expression of integrin transcripts in human cancer cells -- pt. 2. Functional analysis of integrins -- Functional analysis of the [beta]₂ integrins -- expression of heterologous integrin genes -- Preparing a polyclonal antibody to mouse [beta]₁ integrin with function-blocking activity -- Integrin gene targeting -- Determination of positive and negative regulatory elements -- pt. 3. Integrin function at the cellular level -- A cell-based adhesion assay for the characterization of integrin [alpha]v[beta]₃ antagonists -- Integrins in cell migration -- Models for studying cellular invasion of basement membranes -- Methods for studying anoikis -- Use of the 10-day-old chick embryo model for studying angiogenesis -- Cell shape and integrin signaling regulate the differentiation state of mammary epithelial cells. 
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