RNA--protein interaction protocols /
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| Imprint: | Totowa, N.J. : Humana Press, 1999. |
|---|---|
| Description: | xix, 481 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 118 Methods in molecular biology (Clifton, N.J.) ; v. 118. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845313 |
Table of Contents:
- 1. Labeling and Purification of RNA Synthesized by In Vitro Transcription / Paul A. Clarke
- 2. Joining RNA Molecules with T4 DNA Ligase / Melissa J. Moore
- 3. RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs / Michelle M. Hanna, Lori Bentsen and Michael Lucido / [et al.]
- 4. The Methylene Blue Mediated Photocrosslinking Method for Detection of Proteins that Interact with Double-Stranded RNA / Zhi-Ren Liu and Christopher W. J. Smith
- 5. Probing RNA-Protein Interactions by Psoralen Photocrosslinking / Zhuying Wang and Tariq M. Rana
- 6. Identification and Sequence Analysis of RNA-Protein Contact Sites by N-Terminal Sequencing and MALDI-MS / Bernd Thiede, Henning Urlaub and Helga Neubauer / [et al.]
- 7. RNA Footprinting and Modification Interference Analysis / Paul A. Clarke
- 8. Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes / Arthur Gunzl and Albrecht Bindereif
- 9. Nitrocellulose Filter Binding for Determination of Dissociation Constants / Kathleen B. Hall and James K. Kranz
- 10. Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays / David R. Setzer
- 11. PACE Analysis of RNA-Peptide Interactions / Christopher D. Cilley and James R. Williamson
- 12. Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance / Robert J. Crouch, Makoto Wakasa and Mitsuru Haruki
- 13. An Escherichia coli-Based Genetic Strategy for Characterizing RNA Binding Proteins / Chaitanya Jain
- 14. Screening RNA-Binding Libraries Using a Bacterial Transcription Antitermination Assay / Kazuo Harada and Alan D. Frankel
- 15. In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display / Ite A. Laird-Offringa
- 16. In Vitro Selection of Aptamers from RNA Libraries / Daniel J. Kenan and Jack D. Keene
- 17. Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences / Lucy G. Andrews and Jack D. Keene
- 18. Northwestern Screening of Expression Libraries / Paramjeet S. Bagga and Jeffrey Wilusz
- 19. Screening Expression Libraries with Solution-Based Assay / Philippa J. Webster and Paul M. Macdonald
- 20. An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes / Edward Chu, John C. Schmitz and Jingfang Ju / [et al.]
- 21. Purification and Depletion of RNP Particles by Antisense Affinity Chromatography / Benjamin J. Blencowe and Angus I. Lamond
- 22. Purification of U Small Nuclear Ribonucleoprotein Particles / Berthold Kastner and Reinhard Luhrmann
- 23. Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes / Maurice S. Swanson and Gideon Dreyfuss
- 24. Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing / Akila Mayeda and Adrian R. Krainer
- 25. Mammalian In Vitro Splicing Assays / Akila Mayeda and Adrian R. Krainer
- 26. Yeast Pre-mRNA Splicing Extracts / Stephanie W. Ruby
- 27. Prespliceosome and Spliceosome Isolation and Analysis / Laura A. Lindsey and Mariano A. Garcia-Blanco
- 28. A Yeast Spliceosome Assay / Stephanie W. Ruby
- 29. Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing / Thomas A. Cooper
- 30. In Vivo SELEX in Vertebrate Cells / Thomas A. Cooper
- 31. Purification of SR Protein Splicing Factors / Alan M. Zahler
- 32. Processing mRNA 3' Ends in Vitro / Michael J. Imperiale
- 33. Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay / Fernando J. Salles and Sidney Strickland
- 34. In Vitro Translation Extracts from Tissue Culture Cells / Kazuko Shiroki and Akio Nomoto
- 35. Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes / Jeff Ross.
