Protocols for nucleic acid analysis by nonradioactive probes /
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| Imprint: | Totowa, N.J. : Humana Press, c1994. |
|---|---|
| Description: | xii, 268 p. : ill. ; 23 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 28 Methods in molecular biology (Clifton, N.J.) ; v. 28. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845320 |
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| 245 | 0 | 0 | |a Protocols for nucleic acid analysis by nonradioactive probes / |c edited by Peter G. Isaac. |
| 260 | |a Totowa, N.J. : |b Humana Press, |c c1994. | ||
| 300 | |a xii, 268 p. : |b ill. ; |c 23 cm. | ||
| 336 | |a text |b txt |2 rdacontent |0 http://id.loc.gov/vocabulary/contentTypes/txt | ||
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| 490 | 1 | |a Methods in molecular biology ; |v v. 28 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |g Ch. 1. |t About Nonradioactive Nucleic Acid Detection / |r Peter G. Isaac -- |g Ch. 2. |t Isolation of DNA from Plants / |r Justin Stacey and Peter G. Isaac -- |g Ch. 3. |t Isolation of High-Molecular-Weight DNA from Animal Cells / |r Ian Garner -- |g Ch. 4. |t Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes / |r Justin Stacey and Peter G. Isaac -- |g Ch. 5. |t Isolation of Plant RNA / |r Rachel Hodge -- |g Ch. 6. |t Isolation of Total and PolyA[superscript +] RNA from Animal Cells / |r Ian Garner -- |g Ch. 7. |t Preparation of RNA Gel Blots / |r Rachel Hodge -- |g Ch. 8. |t Preparation of RNA Dot Blots / |r Rachel Hodge -- |g Ch. 9. |t Isolation of Plasmids for the Preparation of Probes / |r Peter G. Isaac and Justin Stacey -- |g Ch. 10. |t Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides / |r Tom McCreery and Tim Helentjaris -- |g Ch. 11. |t Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming / |r Tom McCreery and Tim Helentjaris -- |g Ch. 12. |t Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes / |r Esther N. Signer -- |g Ch. 13. |t Labeling of Double-Stranded DNA Probes with Biotin / |r Angela Karp -- |g Ch. 14. |t Preparation of Horseradish Peroxidase-Labeled Probes / |r Ian Durrant and Timothy Stone -- |g Ch. 15. |t Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP / |r Bronwen M. Harvey, Claire B. Wheeler and Martin W. Cunningham -- |g Ch. 16. |t Labeling of Oligonucleotides with Fluorescein / |r Patricia M. E. Chadwick and Ian Durrant -- |g Ch. 17. |t Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence / |r Tom McCreery and Tim Helentjaris -- |g Ch. 18. |t Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection / |r Esther N. Signer -- |g Ch. 19. |t Hybridization and Detection of Digoxigenin Probes on RNA Blots / |r Elizabeth Davies, Rachel Hodge and Peter G. Isaac -- |g Ch. 20. |t Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence / |r Timothy Stone and Ian Durrant -- |g Ch. 21. |t Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence / |r Bronwen M. Harvey and Claire B. Wheeler -- |g Ch. 22. |t Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection / |r Ian Durrant and Patricia M. E. Chadwick -- |g Ch. 23. |t Preparation of Chromosome Spreads by Root-Tip Meristem Dissection for In Situ Hybridization with Biotin-Labeled Probes / |r Angela Karp -- |g Ch. 24. |t Enzymatic Treatment of Plant Material to Spread Chromosomes for In Situ Hybridization / |r Trude Schwarzacher and Andrew R. Leitch -- |g Ch. 25. |t Use of Biotin-Labeled Probes on Plant Chromosomes / |r Angela Karp -- |g Ch. 26. |t Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes / |r Trude Schwarzacher and J. S. (Pat) Heslop-Harrison -- |g Ch. 27. |t Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ / |r Ilia J. Leitch and J. S. (Pat) Heslop-Harrison -- |g Ch. 28. |t Preparation of Tissue Sections and Slides for mRNA Hybridization / |r Giorgio Terenghi and Julia M. Polak -- |g Ch. 29. |t Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes / |r Giorgio Terenghi and Julia M. Polak -- |g Ch. 30. |t Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes / |r Barry Rosen and Rosa Beddington -- |g Ch. 31. |t PACE (Probe Assay - Chemiluminescence Enhanced): A Magnetic Bead Assay for Noncultural Diagnosis of Gonorrhea / |r Paul A. Granato -- |g Ch. 32. |t Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format / |r E. Patrick Groody -- |g Ch. 33. |t Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide Probes: The Reverse Dot Blot Method / |r Ernest S. Kawasaki and Farid F. Chehab -- |g Ch. 34. |t RAPD Assay: A Novel Technique for Genetic Diagnostics / |r Joseph P. del Tufo and Scott V. Tingey -- |g Ch. 35. |t Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction / |r Jon Kratochvil and Thomas G. Laffler -- |g Ch. 36. |t Nucleic Acid Sequence-Based Amplification (NASBA) / |r Larry Malek, Roy Sooknanan and Jean Compton. |
| 650 | 0 | |a Nucleic acid probes. |0 http://id.loc.gov/authorities/subjects/sh89005128 | |
| 650 | 0 | |a Nucleic acids |x Analysis. |0 http://id.loc.gov/authorities/subjects/sh85093145 | |
| 650 | 1 | 2 | |a Nucleic Acid Probes. |
| 650 | 1 | 2 | |a Nucleic Acids |x analysis. |
| 650 | 7 | |a Nucleic acid probes. |2 fast |0 http://id.worldcat.org/fast/fst01041068 | |
| 650 | 7 | |a Nucleic acids |x Analysis. |2 fast |0 http://id.worldcat.org/fast/fst01041071 | |
| 700 | 1 | |a Isaac, Peter G. |0 http://id.loc.gov/authorities/names/n93068174 |1 http://viaf.org/viaf/27221878 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 28. | |
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