Transgenesis techniques : principles and protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c1993. |
|---|---|
| Description: | xii, 467 p. : ill. ; 23 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 18 Methods in molecular biology (Clifton, N.J.) ; v. 18. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845329 |
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| 245 | 0 | 0 | |a Transgenesis techniques : |b principles and protocols / |c edited by David Murphy, David A. Carter. |
| 260 | |a Totowa, N.J. : |b Humana Press, |c c1993. | ||
| 300 | |a xii, 467 p. : |b ill. ; |c 23 cm. | ||
| 336 | |a text |b txt |2 rdacontent |0 http://id.loc.gov/vocabulary/contentTypes/txt | ||
| 337 | |a unmediated |b n |2 rdamedia |0 http://id.loc.gov/vocabulary/mediaTypes/n | ||
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| 490 | 1 | |a Methods in molecular biology ; |v v. 18 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 2 | 0 | |g Ch. 1. |t Introduction to Transgenesis / |r David Murphy and David A. Carter -- |g Ch. 2. |t Transgenic Rodents and the Study of the Central Nervous System / |r David A. Carter -- |g Ch. 3. |t Transgenic Animals and the Study of Cancer / |r David Murphy -- |g Ch. 4. |t Transgenic Animals and the Study of the Immune System / |r Kam M. Hui -- |g Ch. 5. |t The Commercial and Agricultural Applications of Animal Transgenesis / |r Kevin A. Ward and Colin D. Nancarrow -- |g Ch. 6. |t P Element-Mediated Germ-Line Transformation of Drosophila / |r Mark O'Connor and William Chia -- |g Ch. 7. |t Gene Transfer by Microinjection in the Zebra Fish Brachydanio rerio / |r Hong-Woo Khoo, Lay-Hong Ang and Hueh-Bin Lim -- |g Ch. 8. |t Transgenic Induction in Salmonid and Tilapia Fish / |r Norman Maclean -- |g Ch. 9. |t An Overview of Transgenic Mouse Production / |r David Murphy and David A. Carter -- |g Ch. 10. |t Transgene Design / |r David Murphy, David A. Carter and Duncan R. Smith -- |g Ch. 11. |t Isolation of DNA Fragments for Microinjection / |r Duncan R. Smith and David Murphy -- |g Ch. 12. |t Establishing a Colony for Efficient Production of Transgenic Mice / |r David A. Carter -- |g Ch. 13. |t Mating Mice / |r David Murphy -- |g Ch. 14. |t Anesthetizing Mice / |r David A. Carter -- |g Ch. 15. |t Vasectomizing a Mouse / |r David Murphy -- |g Ch. 16. |t Preparation of Culture Media for Fertilized One-Cell Mouse Eggs / |r David A. Carter -- |g Ch. 17. |t Collection of Fertilized One-Cell Mouse Eggs for Microinjection / |r David A. Carter -- |g Ch. 18. |t Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs I: Manual Injection / |r David Murphy -- |g Ch. 19. |t Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs II: Automatic Injection / |r David Murphy -- |g Ch. 20. |t Delivery of Microinjected Eggs to Surrogate Mothers by Oviduct Transfer / |r David A. Carter -- |g Ch. 21. |t Caesarean Section and Fostering / |r David Murphy -- |g Ch. 22. |t In Vitro Fertilization of Mouse Eggs / |r David A. Carter -- |g Ch. 23. |t The In Vitro Isolation of Murine Embryonic Stem Cells / |r Dave Wells -- |g Ch. 24. |t Production of Chimeras Derived from Murine Embryonic Stem Cells / |r Dave Wells -- |g Ch. 25. |t Cryopreservation of Transgenic Mouse Lines / |r Kimball O. Pomeroy -- |g Ch. 26. |t Introduction to the Physiology and Husbandry of the Rat / |r Chiew-Hun Phang -- |g Ch. 27. |t Mating of Rats / |r Chiew-Hun Phang -- |g Ch. 28. |t Vasectomy of the Male Rat / |r Chiew-Hun Phang -- |g Ch. 29. |t Harvest of Fertilized One-Cell Rat Eggs / |r Chiew-Hun Phang -- |g Ch. 30. |t Implantation of Microinjected Eggs / |r Chiew-Hun Phang -- |g Ch. 31. |t Cryopreservation of Transgenic Rat Lines / |r Kimball O. Pomeroy -- |g Ch. 32. |t Production of Transgenic Sheep / |r Colin D. Nancarrow, James T. A. Marshall and Kevin A. Ward -- |g Ch. 33. |t Cryopreservation of Transgenic Sheep Lines / |r Kimball O. Pomeroy -- |g Ch. 34. |t Isolation of Genomic DNA from Tail Tissue / |r David Murphy -- |g Ch. 35. |t Slot Blotting of Genomic Tail DNA / |r David Murphy -- |g Ch. 36. |t Genomic Analysis of Transgenic Animals by the Polymerase Chain Reaction / |r Cathy Abbott -- |g Ch. 37. |t Genomic Analysis of Transgenic Animals: Southern Blotting / |r Duncan R. Smith and David Murphy -- |g Ch. 38. |t The Preparation of RNA from the Tissues of Transgenic Animals / |r David Murphy -- |g Ch. 39. |t Northern Blotting / |r David Murphy -- |g Ch. 40. |t RNA Slot Blotting / |r David Murphy -- |g Ch. 41. |t Analysis of Gene Expression by PCR / |r Peter Koopman -- |g Ch. 42. |t Nuclear Run-On Analysis of Transcription / |r David Murphy -- |g Ch. 43. |t S1 Nuclease Protection Mapping / |r Duncan R. Smith -- |g Ch. 44. |t Primer Extension / |r Duncan R. Smith -- |g Ch. 45. |t In Situ Hybridization Analysis of Transgenic Tissues / |r Judith McNiff Funkhouser -- |g Ch. 46. |t Immunohistochemical Analysis of Transgene Expression / |r Judith McNiff Funkhouser -- |g Ch. 47. |t Postmortem Examination of Transgenic Mice / |r Kum Fai Chooi -- |g Ch. 48. |t Histological Examination of Transgenic Mice / |r Kum Fai Chooi -- |g Ch. 49. |t Reporter Enzyme Assays / |r Karen Pardy -- |g Ch. 50. |t Restriction Endonuclease Digestion of DNA / |r Duncan R. Smith -- |g Ch. 51. |t Agarose Gel Electrophoresis / |r Duncan R. Smith -- |g Ch. 52. |t Working with Radioactivity in the Transgenic Animal Laboratory / |r Duncan R. Smith -- |g Ch. 53. |t Random Primed Labeling of DNA / |r Duncan R. Smith -- |g Ch. 54. |t Filter Hybridization / |r David Murphy -- |g Ch. 55. |t DNA Transfection / |r Karen Pardy. |
| 650 | 0 | |a Animal genetic engineering |x Technique. | |
| 650 | 0 | |a Transgenic animals. |0 http://id.loc.gov/authorities/subjects/sh89007259 | |
| 650 | 0 | |a Genetic transformation |x Technique. | |
| 650 | 2 | |a Animals, Genetically Modified. | |
| 650 | 2 | |a Genetic Engineering |x methods. | |
| 650 | 2 | |a Genetic Engineering |x veterinary. | |
| 650 | 2 | |a Transformation, Genetic. | |
| 650 | 7 | |a Animal genetic engineering |x Technique. |2 fast |0 http://id.worldcat.org/fast/fst00809195 | |
| 650 | 7 | |a Genetic transformation |x Technique. |2 fast |0 http://id.worldcat.org/fast/fst00940105 | |
| 650 | 7 | |a Transgenic animals. |2 fast |0 http://id.worldcat.org/fast/fst01154666 | |
| 700 | 1 | |a Murphy, David, |d 1957- |0 http://id.loc.gov/authorities/names/n93008714 |1 http://viaf.org/viaf/48433324 | |
| 700 | 1 | |a Carter, David A. |q (David Allan), |d 1954- |0 http://id.loc.gov/authorities/names/n93008723 |1 http://viaf.org/viaf/105096189 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 18. | |
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| 903 | |a HeVa | ||
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| 927 | |t Library of Congress classification |a QH506.M45 v.18 |l ASR |c ASR-SciASR |g Analytic |e CRERAR |b A38874429 |i 7962972 | ||
