New protein techniques /
Saved in:
| Imprint: | Clifton, N.J. : Humana Press, c1988. |
|---|---|
| Description: | xv, 531 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Biological methods Methods in molecular biology ; v. 3 Methods in molecular biology (Clifton, N.J.) ; v. 3. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5845340 |
Table of Contents:
- Prevention of unwanted proteolysis / Robert J. Beynon
- The Bradford method for protein quantitation / John B.W. Hammond, Nicholas J. Kruger
- Amino acid analysis by precolumn derivatization ; Identification of N-terminal amino acids by high-performance liquid chromatography / E.L.V. Harris
- Enzymatic methods for cleaving proteins ; Chemical cleavage of proteins / Bryan John Smith
- Chemical modification of proteins / Alex F. Carne
- The design, preparation, and use of immunopurification reagents / Andrew Kenney, Lynne Goulding, Christopher Hill
- Dye-ligand chromatography / Sarojani Angal
- Aminohexyl-Sepharose affinity chromatography : purification of an auxin receptor / R.D.J. Barker, H.M. Bailey
- Purification of DNA-dependent RNA polymerase from eubacteria / N.W. Scott
- Direct immunoprecipitation of protein / Christopher F. Thurston, Lucy F. Henley
- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique / Ketan Patel, David J. Easty, Michael J. Dunn
- Chromatofocusing / Ake Sidén, Paolo Gallo
- Hybrid isoelectric focusing using mixed synthetic-carrier ampholyte-immobilized pH gradient gels ; Two-dimensional electrophoresis using immobilized pH gradients in the first dimension ; Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension / Michael J. Dunn, Ketan Patel
- Preparative aspects of immobilized pH gradients / Pier Giorgio Righetti, Cecilia Gelfi
- Isoelectric focusing under denaturing conditions / Christopher F. Thurston, Lucy F. Henley
- Computer analysis of 2-D electrophoresis gels : image analysis, data base, and graphic aids / H. H-S. Ip
- Two-dimensional (crossed) immunoelectrophoresis / John M. Walker
- Peptide synthesis ; Synthesis of a series of analogous peptides using T-bags / Brian Austen
- Production of antisera to synthetic peptides / W.J. Gullick
- Production of antibodies using protein in gel bands / S.A. Amero, T.C. James, S.C.R. Elgin
- Purification of immunoglobulins using protein A-Sepharose / Nicholas J. Kruger, John B.W. Hammond
- Antibody-enzyme conjugate formation / G.B. Wisdom
- Vacuum blotting : an inexpensive, flexible, qualitative blotting technique ; Blotting with plate electrodes / Marnix Peferoen
- Use of dried milk for immunoblotting / Rosemary Jagus, Jeffrey W. Pollard
- Immunodetection of proteins on "Western" blots using ¹²⁵I-labeled protein A / Nicholas J. Kruger, John B.W. Hammond
- Detection of protein blots using the avidin-biotin system / Michael J. Dunn, Ketan Patel
- Detection of protein blots using enzyme-linked second antibodies or protein A / J.M. Walker, W. Gaastra
- Colloidal gold for the detection of proteins on blots and immunoblots / Alan Jones, Marc Moeremans
- Enzyme immobilization by adsorption ; Enzyme immobilization by entrapment ; Enzyme immobilization by covalent bonding ; Cell immobilization / M.D. Trevan.
