Molecular genetics of bacteria /
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| Author / Creator: | Snyder, Larry. |
|---|---|
| Edition: | 3rd ed. |
| Imprint: | Washington, D.C. : ASM Press, c2007. |
| Description: | xvii, 735 p. : ill. ; 29 cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/6420716 |
Table of Contents:
- Introduction
- The Biological Universe
- What Is Genetics?
- Bacterial Genetics
- Phage Genetics
- A Brief History of Bacterial Molecular Genetics
- What's Ahead
- 1. The Bacterial Chromosome: DNA Structure, Replication, and Segregation
- DNA Structure
- The Mechanism of DNA Replication
- Replication Errors
- Replication of the Bacterial Chromosome and Cell Division
- The Bacterial Nucleoid
- The Bacterial Genome
- Antibiotics That Affect Replication and DNA Structure
- Molecular Biology Manipulations with DNA
- Box 1.1. Linear Chromosomes in Bacteria
- Box 1.2. Restarting Replication Forks
- Box 1.3. The Bacterial Cytoskeleton and Bacterial Cell Biology
- Box 1.4. Features of Bacterial Genomes
- Box 1.5. Bacterial Genome Sequencing
- 2. Bacterial Gene Expression: Transcription, Translation, and Protein Folding
- Overview
- The Structure and Function of RNA
- Transcription
- Proteins
- Protein Folding
- Membrane Proteins and Protein Export
- Introns and Inteins
- Useful Concepts
- Antibiotics That Block Transcription and Translation
- Box 2.1. Molecular Phylogeny
- Box 2.2. Mimicry in Translation
- Box 2.3. Exceptions to the Code
- Box 2.4. Traffic Jams on mRNA: Removing Stalled Ribosomes with tmRNA
- Box 2.5. Stability and Degradation of mRNA
- Box 2.6. Selfish DNAs: RNA Introns and Protein Inteins
- Box 2.7. Annotation and Comparative Genomics
- 3. Bacterial Genetic Analysis: Forward and Reverse
- Definitions
- Useful Phenotypes in Bacterial Genetics
- Inheritance in Bacteria
- Mutation Rates
- Types of Mutations
- Reversion versus Suppression
- Genetic Analysis in Bacteria
- Gene Replacements and Reverse Genetics
- Isolation of Tandem Duplications of the his Operon in Salmonella enterica Serovar Typhimurium
- Box 3.1. Statistical Analysis of the Number of Mutants per Culture
- Box 3.2. Inversions and the Genetic Map
- 4. Plasmids
- What Is a Plasmid?
- Properties of Plasmids
- Constructing a Plasmid Cloning Vector
- Box 4.1. Plasmids and Bacterial Pathogenesis
- Box 4.2. Linear Plasmids
- Box 4.3. An Incompatibility Group of One's Own
- Box 4.4. Plasmid Addiction
- 5. Conjugation
- Overview
- Mechanism of DNA Transfer during Conjugation in Gram-Negative Bacteria
- Chromosome Transfer by Plasmids
- Transfer Systems of Gram-Positive Bacteria
- Other Types of Transmissible Elements
- Box 5.1. Gene Exchange between Kingdoms
- Box 5.2. Conjugation and Type IV Protein Secretion Systems
- Box 5.3. Conjugation in Streptomycetes
- Box 5.4. Conjugative Transposons (Integrating Conjugative Elements)
- 6. Transformation
- Natural Transformation
- Importance of Natural Transformation for Forward and Reverse Genetics
- Artificially Induced Competence
- Box 6.1. Antigenic Variation in Neisseria gonorrhoeae
- 7. Lytic Bacteriophages: Development, Genetics, and Generalized Transduction
- The Bacteriophage Lytic Development Cycle
- Phage DNA Replication
- Phage Lysis
- Genetic Analysis of Phages
- Generalized Transduction
- Box 7.1. RNA Phages
- Box 7.2. Phage Display
- Box 7.3. Protein Priming
- 8. Lysogeny: the ? Paradigm and the Role of Lysogenic Conversion in Bacterial Pathogenesis
- Phage ?
- Replication of ? DNA
- Lysogeny
- Specialized Transduction
- Other Lysogen-Forming Phages
- Lysogenic Conversion and Bacterial Pathogenesis
- Genetic Experiments with Phage ?
- Box 8.1. Effects of Prophage Integration on the Host
- Box 8.2. Retroregulation
- Box 8.3. How a Pathogenicity Island Gets Around
- 9. Transposition, Site-Specific Recombination, and Families of Recombinases
- Transposition
- Mechanisms of Transposition
- Details of Transposition by the DDE Transposons
- Rolling-Circle Transposons
- Y and S Transposons
- General Properties of Transposons
- Transposon Mutagenesis
- Site-Specific Recombination
- Y and S Recombinases
- Importance of Transposition and Site-Specific Recombination in Bacterial Adaptation
- Box 9.1. Phage Mu: a Transposon Masquerading as a Phage
- Box 9.2. Transposon Mutagenesis In Vitro
- 10. Molecular Mechanisms of Homologous Recombination
- Overview of Recombination
- Molecular Models of Recombination
- The Molecular Basis for Recombination in E. coli
- Phage Recombination Pathways
- Genetic Analysis of Recombination in Bacteria
- Box 10.1. Breaking and Entering: Introns and Inteins Move by Double-Strand Break Repair or Retrohoming
- Box 10.2. The Three R's: Recombination, Replication, and Repair
- Box 10.3. Recombineering: Gene Replacements in E. coli with Phage ? Recombination Functions
- 11. DNA Repair and Mutagenesis
- Evidence for DNA Repair
- Specific Repair Pathways
- General Repair Mechanisms
- DNA Damage Tolerance Mechanisms
- Summary of Repair Pathways in E. coli
- Bacteriophage Repair Pathways
- Box 11.1. Oxygen: the Enemy Within
- Box 11.2. Cancer and Mismatch Repair
- Box 11.3. Transcription-Repair Coupling
- Box 11.4. Translesion Synthesis and Cancer
- Box 11.5. The Ames Test
- 12. Regulation of Gene Expression: Operons
- Transcriptional Regulation in Bacteria
- Negative Transcriptional Regulation
- Positive Regulation
- Regulation by Attenuation of Transcription
- Posttranslational Regulation: Feedback Inhibition
- Operon Analysis for Sequenced Genomes
- Box 12.1. The Helix-Turn-Helix Motif of DNA-Binding Proteins
- Box 12.2. Families of Regulators
- 13. Global Regulation: Regulons and Stimulons
- Catabolite-Sensitive Operons
- Regulation of Nitrogen Assimilation
- Stress Responses in Bacteria
- Extracytoplasmic (Envelope) Stress Responses
- Iron Regulation in E. coli
- Regulation of Virulence Genes in Pathogenic Bacteria
- Regulation of Ribosome and tRNA Synthesis
- Microarray and Proteomic Analysis of Regulatory Networks
- Box 13.1. cAMP-Independent Catabolite Repression
- Box 13.2. Nitrogen Fixation
- Box 13.3. Sigma Factors
- Box 13.4. Signal Transduction Systems in Bacteria
- Box 13.5. Regulatory RNAs
- 14. Bacterial Cell Compartmentalization and Sporulation
- Analysis of Protein Transport in Escherichia coli
- Genetic Analysis of Transmembrane Domains of Inner Membrane Proteins in Gram-Negative Bacteria
- Protein Secretion
- Genetic Analysis of Sporulation in Bacillus subtilis
- Box 14.1. Secretion Systems and Motility
- Box 14.2. Phosphorelay Activation of the Transcription Factor Spo0A
- Answers to Questions for Thought and Problems
- Glossary
- Figure and Table Credits
- Index
