Protein targeting protocols /
Saved in:
| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, c2007. |
| Description: | xv, 501 p. : ill. (some col.) ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; 390 Methods in molecular biology (Clifton, N.J.) ; v. 390. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/6422426 |
Table of Contents:
- Preface
- Contributors
- Part I. Bacterial Systems
- 1. Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching
- 2. Membrane Protein Insertion and Secretion in Bacteria
- 3. Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria
- 4. Periplasmic Targeting of Recombinant Proteins in Escherichia coli
- 5. In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export
- Part II. Eukaryotic Systems
- 6. Nuclear Import Properties of the Sex-Determining Factor SRY
- 7. The Mitochondrial Machinery for Import of Precursor Proteins
- 8. A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells
- 9. In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria
- 10. Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa: In Vitro and In Vivo Studies
- 11. A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70
- 12. The Chloroplast Protein Import Machinery: A Review
- 13. Import of Plastid Precursor Proteins Into Pea Chloroplasts
- 14. Protein Targeting in "Secondary" or "Complex" Chloroplasts
- 15. Protein Trafficking to the Complex Chloroplasts of Euglena
- 16. Fluorescent Protein Fusions for Protein Localization in Plants
- 17. Genetic Transformation: A Tool to Study Protein Targeting in Diatoms
- 18. Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening
- 19. Trafficking Through the Early Secretory Pathway of Mammalian Cells
- 20. Studying Protein Export From the Endoplasmic Reticulum in Plants
- 21. Imaging the Golgi Apparatus in Living Mitotic Cells
- 22. Evaluating Yeast Biosynthetic Vacuolar Transport
- 23. Targeting to Lysosomes in Mammalian Cells: The Biosynthetic and Endocytic Pathways
- 24. Monitoring Autophagy in Yeast: The Pho8[Delta]60 Assay
- 25. Protein Targeting to Yeast Peroxisomes
- 26. Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins
- Part III. General Methods
- 27. Intracellular Protein Localization by Immunoelectron Microscopy
- 28. Separation of Proteins by Blue Native Electrophoresis
- 29. Computational Prediction of Subcellular Localization
- 30. Phylogenetic Analysis to Uncover Organellar Origins of Nuclear-Encoded Genes
- Index
