Integrated biochips for DNA analysis /
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| Imprint: | Austin, Tex. : Landes Bioscience ; New York : Springer Science+Business Media, c2007. |
|---|---|
| Description: | 201 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Biotechnology intelligence unit Biotechnology intelligence unit (Unnumbered) |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/6834503 |
Table of Contents:
- Preface
- 1. Integrated DNA Biochips: Past, Present and Future
- Early Developments
- Formative Years
- New Developments
- Future and Path Forward
- 2. Integrated Molecular Analyses of Biological Samples on a Bead-Based Microarray Platform
- Universal Arrays and the GoldenGate Genotyping Assay
- Gene Expression Analysis with the DASL Assay
- Allele-Specific Expression Analysis
- DNA Methylation Analysis
- Whole-Genome Gene Expression
- Whole-Genome Genotyping
- 3. Integrated Microfluidic CustomArray Biochips for Gene Expression and Genotyping Analysis
- Semiconductor-Based DNA Microarray
- Microfluidic Cartridge
- Fluidic Architecture and Operation
- Micropumps
- Micromixing
- Microvalves
- Gene Expression Assay
- Subtyping Assay
- Sequencing Assay
- Discussion
- 4. Self-Contained, Fully Integrated Biochips for Sample Preparation, PCR Amplification and DNA Microarray Analysis
- Device Design and Fabrication
- Fluidic Transport
- Micromixing
- Microvalves
- Micropumps
- Sample Preparation
- On-Chip DNA Amplification
- DNA Microarray Detection
- Pathogenic Bacteria Detection
- Single Nucleotide Polymorphism Assay
- Discussion
- Conclusion
- 5. Integrating Sample Processing and Detection with Microchip Capillary Electrophoresis of DNA
- Microchip CE of DNA
- Integrating Sample Processing with Microchip CE of DNA
- Integrated DNA Detectors for CE Microchips
- Phase-Changing Sacrificial Materials for Polymer Microchip Analysis Systems
- 6. Integrated Plastic Microfluidic Devices for Bacterial Detection
- Device Design and Fabrication
- Microfluidic Valves
- Screen-Printed Heaters and Electrodes
- PCR Thermal Cycling
- Bacterial Detection
- 7. PCR in Integrated Microfluidic Systems
- PCR in Microfluidic Systems
- Integrated PCR and Gel Electrophoresis
- Integrated PCR and Sample Purification
- Integrated PCR and Hybridization
- Further Advancements in Integration
- Novel Micro-PCR Methods
- 8. Integrated Nucleic Acid Analysis in Parallel Matrix Architecture
- Nucleic Acid Analysis
- Pneumatic Control of Microfluid
- Integration and Parallelization on a Microfluidic Chip
- DNA Analyses with Complex Parallelization
- RNA Analyses
- Matrix PCR on a Microfluidic Chip
- Pico Titer Plate-Based PCR
- Microfluidic Digital PCR
- Microfluidic Sanger DNA Sequencing
- Microfluidic DNA Sequencing-by-Synthesis
- Summary and Perspective
- 9. Chip-Based Genotyping by Mass Spectrometry
- MALDI Based Genotyping Methods
- Chip as a Sample Positioning and Concentration Device
- Chip as a Functionalized Capture Device
- ESI Based Genotyping Methods
- 10. Analyzing DNA-Protein Interactions on a Chip
- Approaches to Identify Cis-Regulatory Elements
- Surface Plasmon Resonance-Based Assays
- Using Surface Plasmon Resonance to Measure DNA-Protein Interaction
- Tests Using a Spreeta Biosensor
- Experimental Set Up
- Measuring DNA-Protein Interactions Using the Surface Plasmon Resonance Sensor
- Surface Preparation
- Measurements of Double-Stranded DNA Surface Adsorption
- Sensitivity of the Surface Plasmon Resonance Based Sensor
- Projected Sensitivity Based on Preliminary Experiments
- Surface Plasmon Resonance Is Sensitive Enough to Detect Physiologically Relevant DNA-Protein Interactions
- 11. Single Molecule DNA Detection
- Single-Molecule DNA Detection Based on Use of Molecular FRET Probes
- Single-Quantum-Dot-Based FRET Nanosensor for DNA Detection
- Single-Molecule Fluorescence Burst Coincidence Detection
- Multiplexed DNA Detection Based on Multicolor Colocalization Analysis of Quantum Dot Nanoprobes
- Manipulation and Focusing of Single Molecules
- Conclusion and Prospectives
- 12. Nanochannels for Genomic DNA Analysis: The Long and the Short of It
- Theory
- Methods: Realizations of Nanochannels
- Experimental Results
- 13. Beyond Microtechnology-Nanotechnology in Molecular Diagnosis
- History of Nanotechnology
- Safety and Nanotechnology
- Nanofabrication Methods
- Selected Applications in Molecular Diagnosis
- Index
