RNA-protein interaction protocols /
Saved in:
| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana ; London : Springer [distributor], 2008. |
| Description: | xv, 416 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 488 Methods in molecular biology (Clifton, N.J.) ; v. 488. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/7359251 |
Table of Contents:
- Preface
- Contributors
- 1. Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RNA Affinity Chromatography
- 2. An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase
- 3. RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNA-Protein Complexes
- 4. Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
- 5. Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques
- 6. CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins
- 7. Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift
- 8. Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
- 9. Characterization of RNA-Protein Interactions by Phosphorothioate Footprinting and Its Applications to the Ribosome
- 10. In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
- 11. T7 RNA Polymerase-Mediated Incorporation of 8-N[subscript 3]AMP Into RNA for Studying Protein-RNA Interactions
- 12. A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein
- 13. Proteins Specifically Modified With a Chemical Nuclease as Probes of RNA-Protein Interaction
- 14. RNA-Protein Crosslink Mapping Using TEV Protease
- 15. Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
- 16. Analyzing RNA-Protein Crosslinking Sites in Unlabeled Ribonucleoprotein Complexes by Mass Spectrometry
- 17. In Vitro Selection of Random RNA Fragments to Identify Protein-Binding Sites Within Large RNAs
- 18. Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes
- 19. Mapping the Regions of RNase P Catalytic RNA That Are Potentially in Close Contact With Its Protein Cofactor
- 20. Quantification of MicroRNAs, Splicing Isoforms, and Homologous mRNAs With the Invader Assay
- 21. Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transferase-Dependent PCR
- 22. Duplex Unwinding and RNP Remodeling With RNA Helicases
- 23. Preparation of Efficient Splicing Extracts From Whole Cells, Nuclei, and Cytoplasmic Fractions
- 24. Designing and Utilization of siRNAs Targeting RNA Binding Proteins
- 25. The Use of Saccharomyces cerevisiae Proteomic Libraries to Identify RNA-Modifying Proteins
- Index
