Candida albicans : methods and protocols /
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| Imprint: | New York, NY : Humana Press, c2009. |
|---|---|
| Description: | x, 206 p. : ill. ; 27 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; 499 Methods in molecular biology (Clifton, N.J.) ; v. 499. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/7639445 |
Table of Contents:
- Preface
- Contributors
- Part I. Immunogical Methods
- 1. Isolation of Dendritic Cells from Human Blood for In Vitro Interaction Studies with Fungal Antigens
- 2. Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluids
- 3. Phagocytosis and Killing Assays for Candida Species
- 4. Immunization Protocols for Use in Animal Models of Candidiasis
- Part II. Virulence and Biofilms
- 5. Penetration of Antifungal Agents Through Candida Biofilms
- 6. Candida Biofilm Analysis in the Artificial Throat Using Fish
- 7. Conditions for Optimal Candida Biofilm Development in Microtiter Plates
- Part III. Virulence Measurements: In Vitro, Ex Vivo, and In Vivo
- 8. Animal Models of Candidiasis
- 9. Candida albicans Gene Expression in an In Vivo Infection Model
- 10. In Vitro and Ex Vivo Assays of Virulence in Candida albicans
- Part IV. Strain Typing and Identification
- 11. Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity
- 12. DNA Fingerprinting Candida Species
- Part V. Genomics and Proteomics
- 13. The Application of Tandem-Affinity Purification to Candida albicans
- 14. Preparation of Samples for Proteomic Analysis of the Candida albicans Cell Wall
- 15. Reporter Gene Assays in Candida albicans
- 16. Genetic Transformation of Candida albicans
- 17. Large-Scale Gene Disruption Using the UAUI Cassette
- Part VI. Appendix
- 18. Standard Growth Media and Common Techniques for Use with Candida albicans
- Index
