Extracellular matrix protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | New York , NY : Humana Press ; London : Springer [distributor], c2009. |
| Description: | xi, 375 p. : ill. (some col.) ; 27 cm. |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; 522 Methods in molecular biology (Clifton, N.J.) ; v. 522. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/7697442 |
Table of Contents:
- Preface
- Contributors
- Part I. Molecular Biology
- 1. Retroviral Delivery of ECM Genes
- 2. Tissue-Specific KO of ECM Proteins
- 3. Recombinant Collagen Trimers from Insect Cells and Yeast
- 4. Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag
- 5. Preparation of Recombinant Fibronectin Fragments for Functional and Structural Studies
- Part II. Biochemical and Biophysical Analysis
- 6. Quantitative Determination of Collagen Cross-links
- 7. ECM Macromolecules: Height-Mapping and Nano-Mechanics Using Atomic Force Microscopy
- 8. Atomic Force Microscopy Measurements of Intermolecular Binding Forces
- 9. Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy
- 10. Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry
- 11. ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy
- 12. Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates
- 13. Solid Phase Assays for Studying ECM Protein-Protein Interactions
- Part III. Cell Biology Assays
- 14. Cell Adhesion Assays
- 15. ECM Degradation Assays for Analyzing Local Cell Invasion
- 16. Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration
- 17. Fibrin Gel Model for Assessment of Cellular Contractility
- 18. Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis)
- 19. Stromagenesis During Tumorigenesis: Characterization of Tumor-Associated Fibroblasts and Stroma-Derived 3D Matrices
- Part IV. Organ Models
- 20. Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
- 21. ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development
- 22. Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice
- Part V. Tissue Engineering
- 23. Characterizing ECM Production by Cells Encapsulated in Hydrogels
- 24. Tissue Engineering and Cell-Populated Collagen Matrices
- Index
