Mitochondrial DNA : methods and protocols /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | New York : Humana Press, c2009. |
| Description: | xi, 403 p. : ill. ; 27 cm. |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; v. 554 Methods in molecular biology (Clifton, N.J.) ; v. 554. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/7847698 |
Table of Contents:
- Preface
- Contributors
- Part I. Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly
- 1. Biochemical Isolation of mtDNA Nucleoids from Animal Cells
- 2. Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophorsis
- 3. Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins: DNA Polymerase ¿ and Mitochondrial Single-Stranded DNA-Binding Protein
- 4. Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
- 5. Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein
- 6. Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
- 7. Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Helicase Twinkle
- 8. Purification Strategy for Recombinant Forms of the Human Mitochondrial DNA Helicase
- 9. Methods for Studying Mitochondrial Transcription Termination with Isolated Components
- 10. Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes
- Part II. Mitochondrial DNA Damage And Repair
- 11. Reactive Oxygen Species Production by Mitochondria
- 12. Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
- 13. Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method
- 14. In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria
- 15. Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction
- 16. Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nuclear, Oxidative DNA Damage
- Part III. Mitochondrial DNA Mutations
- 17. Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae
- 18. Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis
- 19. Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease
- 20. Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells
- 21. Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR
- 22. Measuring DNA Precursor Pools in Mitochondria
- 23. Establishment of Human Cell Lines Lacking Mitochondrial DNA
- Index
