Protein purification : principles, high resolution methods, and applications /

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Bibliographic Details
Edition:3rd ed.
Imprint:Hoboken, N.J. : John Wiley & Sons, c2011.
Description:xiv, 517 p. : ill. (some col.) ; 29 cm.
Language:English
Series:Wiley series in methods of biochemical analysis ; v. 54
Methods of biochemical analysis ; v. 54.
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/8508843
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Other authors / contributors:Janson, Jan-Christer.
ISBN:9780471746614 (cloth)
0471746614 (cloth)
Notes:Includes bibliographical references and index.
Description
Summary:

The authoritative guide on protein purification--now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

High-resolution reversed-phase liquid chromatography

Electrophoresis in gels

Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

Affinity ligands from chemical and biological combinatorial libraries

Membrane separations

Refolding of inclusion body proteins from E. coli

Purification of PEGylated proteins

High throughput screening techniques in protein purification

The history of protein chromatography

Physical Description:xiv, 517 p. : ill. (some col.) ; 29 cm.
Bibliography:Includes bibliographical references and index.
ISBN:9780471746614
0471746614