Protein purification : principles, high resolution methods, and applications /
Saved in:
| Edition: | 3rd ed. |
|---|---|
| Imprint: | Hoboken, N.J. : John Wiley & Sons, c2011. |
| Description: | xiv, 517 p. : ill. (some col.) ; 29 cm. |
| Language: | English |
| Series: | Wiley series in methods of biochemical analysis ; v. 54 Methods of biochemical analysis ; v. 54. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/8508843 |
Table of Contents:
- Preface
- Contributors
- Part I. Introduction
- 1. Introduction to Protein Purification
- Part II. Chromatography
- 2. Introduction to Chromatography
- 3. Gel Filtration
- 4. Ion Exchange Chromatography
- 5. High-Resolution Reversed-Phase Chromatography
- 6. Hydrophobic Interaction Chromatography
- 7. Immobilized Metal Ion Affinity Chromatography
- 8. Covalent Chromatpgraphy
- 9. Affinity Chromatography
- 10. Affinity Ligands from Chemical Combinatorial Libraries
- 11. Affinity Ligands from Biological Combinatorial Libraries
- Part III. Other Separation Methods and Related Techniques
- 12. Membrane Separations
- 13. Refolding of Inclusion Body Proteins from E. coli
- 14. Purification of PEGylated Proteins
- Part IV. Electrophoresis
- 15. Electrophoresis in Gels
- 16. Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients
- 17. Two-Dimensional Electrophoresis in Proteomics
- 18. Protein Elution and Blotting Techniques
- 19. Capillary Electrophoretic Separations
- Part V. Separation Method Optimization
- 20. High Throughput Screening Techniques on Protein Purification
- Index
