Ion-exchange chromatography of proteins /
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| Author / Creator: | Yamamoto, Shūichi, 1953- |
|---|---|
| Imprint: | New York : M. Dekker, c1988. |
| Description: | vii, 401 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Chromatographic science v. 43 |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/906129 |
Table of Contents:
- Preface
- 1. Introduction
- 1.1. Terminology
- 1.2. Basic Concepts of Column Chromatography
- 1.3. Brief History of Ion-Exchange Chromatography
- 1.4. Comparison of Ion-Exchange Chromatography with Other Chromatographic Techniques
- 1.5. Characteristics of Ion-Exchange Chromatography of Proteins
- 1.6. Various Elution Procedures of Ion-Exchange Chromatography of Proteins
- 2. Theoretical Aspects
- 2.1. General Theories of Chromatography
- 2.2. Theory of Ion-Exchange Chromatography of Proteins
- 3. Ion-Exchange Equilibria
- 3.1. Theoretical Treatment
- 3.2. Experimental Methods
- 3.3. Experimental Data
- 4. Diffusion in the Ion Exchanger
- 4.1. Theoretical Treatment
- 4.2. Experimental Methods
- 4.3. Experimental Data
- 5. Axial Dispersion in the Ion-Exchange Column
- 6. Experimental Methods and Apparatus
- 6.1. Apparatus
- 6.2. Pretreatment
- 6.3. Preparation of Ion-Exchange Gel Columns
- 6.4. Method for Sample Introduction
- 6.5. Method for Elution
- 6.6. Regeneration of the Column
- 6.7. Concentration of Fractions
- 6.8. Other Subjects
- 7. Ion Exchangers
- 8. Factors Affecting Separation Behavior
- 8.1. Elution Behavior in Linear Gradient Elution
- 8.2. Prediction of Peak Position and Peak Width in Linear Gradient Elution by Simplified Methods
- 8.3. Resolution in Linear Gradient Elution
- 8.4. Stepwise Elution
- 8.5. Other Special Separation Methods and Apparatus
- 8.6. Summary and Additional Comments
- 9. Large-Scale Operation
- 9.1. General Considerations
- 9.2. Applications
- 9.3. Problems Encountered in Actual Operation
- 10. Design Calculation Procedure
- 10.1. Linear Gradient Elution
- 10.2. Stepwise Elution
- Concluding Remarks and Future Aspects
- Appendix
- A. Derivation of the Value of K[subscript S]
- B. Derivation of Equation (2.59)
- C. Analytical Solutions for a Change in Ionic Strength in Stepwise Elution and Linear Gradient Elution
- D. Numerical Calculation Procedure
- E. A Solution for the Peak Width in Linear Gradient Elution Under the Quasi-Steady State
- F. Values of the Molecular Diffusion Coefficient and its Estimation Method
- Contents
- Symbols
- References
- Index
