Hidden Bibliographic Details
| Other authors / contributors: | Kapusta, Peter, editor.
Wahl, Michael, editor.
Erdmann, Rainer, 1966- editor.
Ahlrichs, Andreas, contributor.
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| ISBN: | 9783319156361
3319156365
9783319156354
3319156357
9783319156354
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| Digital file characteristics: | text file PDF
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| Notes: | Includes bibliographical references and index.
Print version record.
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| Summary: | This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a ℓ́ℓfingerprintℓ́ℓ for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of F©œrster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
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| Other form: | Print version: Advanced Photon Counting 3319156357
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| Standard no.: | 10.1007/978-3-319-15636-1
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