Quantitative PCR protocols /

Quantitative PCR protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©1999.
Description:	1 online resource (x, 304 pages) : illustrations
Language:	English
Series:	Methods in molecular medicine ; 26 Methods in molecular medicine ; 26.
Subject:	Polymerase chain reaction -- Laboratory manuals. RNA. Methodology. DNA. Polymerase chain reaction. Nucleic acids. Genetics -- Technique. RNA Methods DNA Polymerase Chain Reaction Investigative Techniques Nucleic Acid Amplification Techniques Nucleic Acids Nucleic Acids, Nucleotides, and Nucleosides Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Techniques Chemicals and Drugs RNA Nucleic acids Methodology Genetics -- Technique DNA Polymerase chain reaction Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11110265

Hidden Bibliographic Details
Other authors / contributors:	Kochanowski, Bernd. Reischl, Udo.
ISBN:	9781592592623 1592592627 0585239789 9780585239781 1280830514 9781280830518 9786610830510 6610830517 0896035182 9780896035188
Notes:	Includes bibliographical references and index. Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 English. digitized 2010 HathiTrust Digital Library committed to preserve Print version record.
Summary:	In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions.
Other form:	Print version: Quantitative PCR protocols. Totowa, N.J. : Humana Press, ©1999 0896035182 Online version: Quantitative PCR protocols. Totowa, N.J. : Humana Press, ©1999