Hidden Bibliographic Details
| Other authors / contributors: | Chen, Bing-Yuan.
Janes, Harry W.
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| ISBN: | 9781592591770
9781617372810
0585403341
9780585403342
0896039730
9780896039735
1592591779
0896039692
9780896039698
9786610821396
6610821399
1280821396
9781280821394
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| Notes: | Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
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| Summary: | Annotation PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
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| Other form: | Print version: PCR cloning protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2002 0896039692
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| Standard no.: | 10.1385/1592591779.
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