Protein expression : a practical approach /

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Bibliographic Details
Imprint:Oxford ; New York : Oxford University Press, 1999.
Description:1 online resource (xix, 282 pages) : illustrations.
Language:English
Series:The practical approach series ; 202
Practical approach series ; 202.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11127857
Hidden Bibliographic Details
Other authors / contributors:Higgins, S. J. (Steve J.)
Hames, B. D.
ISBN:0585484171
9780585484174
1280374810
9781280374814
9786610374816
6610374813
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
Other form:Print version: Protein expression. Oxford ; New York : Oxford University Press, 1999 0199636230 0199636249