Flow cytometry protocols.

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Bibliographic Details
Edition:2nd ed. / edited by Teresa S. Hawley, Robert G. Hawley.
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (xiii, 434 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1064-3745 ; 263
Methods in molecular biology (Clifton, N.J.) ; v. 263.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128481
Hidden Bibliographic Details
Other authors / contributors:Hawley, Teresa S.
Hawley, Robert G.
ISBN:9781592597734
1592597734
1588292347
9781588292346
1588292355
9781588292353
1280360283
9781280360282
9786610360284
6610360286
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Annotation This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.
Other form:Print version: Flow cytometry protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2004 1588292347 1588292355
Standard no.:10.1385/1592597734