Flow cytometry protocols.

Flow cytometry protocols.

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Bibliographic Details
Edition:	2nd ed. / edited by Teresa S. Hawley, Robert G. Hawley.
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (xiii, 434 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 263 Methods in molecular biology (Clifton, N.J.) ; v. 263.
Subject:	Flow cytometry -- Laboratory manuals. Flow Cytometry -- methods. Flow cytometry. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11128481

Hidden Bibliographic Details
Other authors / contributors:	Hawley, Teresa S. Hawley, Robert G.
ISBN:	9781592597734 1592597734 1588292347 9781588292346 1588292355 9781588292353 1280360283 9781280360282 9786610360284 6610360286
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Annotation This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.
Other form:	Print version: Flow cytometry protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2004 1588292347 1588292355
Standard no.:	10.1385/1592597734

Description
Summary:	Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2-22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23-24).
Physical Description:	1 online resource (xiii, 434 pages) : illustrations (some color)
Bibliography:	Includes bibliographical references and index.
ISBN:	9781592597734 1592597734 1588292347 9781588292346 1588292355 9781588292353 1280360283 9781280360282 9786610360284 6610360286
ISSN:	1064-3745 ;