Flow cytometry protocols.

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Bibliographic Details
Edition:2nd ed. / edited by Teresa S. Hawley, Robert G. Hawley.
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (xiii, 434 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1064-3745 ; 263
Methods in molecular biology (Clifton, N.J.) ; v. 263.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128481
Hidden Bibliographic Details
Other authors / contributors:Hawley, Teresa S.
Hawley, Robert G.
ISBN:9781592597734
1592597734
1588292347
9781588292346
1588292355
9781588292353
1280360283
9781280360282
9786610360284
6610360286
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Annotation This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.
Other form:Print version: Flow cytometry protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2004 1588292347 1588292355
Standard no.:10.1385/1592597734

MARC

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245 0 0 |a Flow cytometry protocols. 
250 |a 2nd ed. /  |b edited by Teresa S. Hawley, Robert G. Hawley. 
260 |a Totowa, N.J. :  |b Humana Press,  |c ©2004. 
300 |a 1 online resource (xiii, 434 pages) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in molecular biology,  |x 1064-3745 ;  |v 263 
504 |a Includes bibliographical references and index. 
505 0 |a Flow cytometry : an introduction / Alice L. Givan -- Multiparameter flow cytometry of bacteria / Howard M. Shapiro and Gerhard Nebe-von-Caron -- Multiparameter data acquisition and analysis of leukocytes / Carleton C. Stewart and Sigrid J. Stewart -- Flow cytometric analysis of kinase signaling cascades / Omar D. Perez, Peter O. Krutzik, and Garry P. Nolan -- Cytokine flow cytometry / Holden T. Maecker -- Use of cell-tracking dyes to determine the proliferation precursor frequencies of antigen-specific t cells / Alice L. Givan [and others] -- Assessment of lymphocyte-mediated cytotoxicity using flow cytometry / Luzheng Liu [and others] -- Multiparametric analysis of apoptosis by flow and image cytometry / William G. Telford, Akira Komoriya, and Beverly Z. Packard -- Detection and enrichment of hematopoietic stem cells by side population phenotype / Shannon S. Eaker [and others] -- Hematopoietic stem cell characterization by hoechst 33342 and rhodamine 123 staining / Ivan Bertoncello and Brenda Williams -- Phenotypic and functional analyses of human CD34neg hematopoietic precursors from mobilized peripheral blood / Douglas C. Dooley and Barbara K. Oppenlander -- Multiparameter flow cytometry of fluorescent protein reporters / Teresa S. Hawley [and others]. 
505 0 |a Analysis of fluorescent protein expressing cells by flow cytometry / Steven C. Pruitt, Lawrence M. Mielnicki, and Carleton C. Stewart -- Integrative flow cytometric and microarray approaches for use in transcriptional profiling / David W. Galbraith, Rangasamy Elumalai, and Fang Cheng Gong -- Flow cytometric analysis of fluorescence resonance energy transfer : a tool for high-throughput screening of molecular interactions in living cells / Francis Ka-Ming Chan and Kevin L. Holmes -- Design of a fluorescence-activated cell sorting based mammalian protein/protein interaction trap / Sam Lievens [and others] -- Flow cytometric screening of yeast surface display libraries / Michael Feldhaus and Robert Siegel -- Fluorescence resonance energy transfer based hiv-1 virion fusion assay -- Marielle cavrois [and others] -- Cell cycle analysis of asynchronous populations / Michael G. Ormerod -- Solid tumor dna content analysis / Adel K. El-Naggar and Philippe Vielh -- Concurrent flow cytometric analysis of DNA and RNA / Adel K. El-Naggar -- Telomere length measurement by fluorescenece in situ hybridiziation and flow cytometry / Veena Kapoor and William G. Telford -- Small lasers in flow cytometry / William G. Telford -- Viable infectious cell sorting in a BSL-3 facility / Stephen P. Perfetto [and others]. 
520 8 |a Annotation  |b This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures. 
588 0 |a Print version record. 
546 |a English. 
650 0 |a Flow cytometry  |v Laboratory manuals. 
650 1 2 |a Flow Cytometry  |x methods.  |0 https://id.nlm.nih.gov/mesh/D005434Q000379 
650 7 |a MEDICAL  |x Histology.  |2 bisacsh 
650 7 |a Flow cytometry.  |2 fast  |0 (OCoLC)fst00927770 
655 4 |a Electronic books. 
655 7 |a Laboratory manuals.  |2 fast  |0 (OCoLC)fst01920776 
700 1 |a Hawley, Teresa S. 
700 1 |a Hawley, Robert G. 
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