Mycobacteria protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©1998.
Description:1 online resource (xiv, 472 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 101
Methods in molecular biology (Clifton, N.J.) ; v. 101.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11128704
Hidden Bibliographic Details
Other authors / contributors:Parish, Tanya.
Stoker, Neil G.
ISBN:0896034712
9780896034716
9781592595761
1592595766
128083689X
9781280836893
9786610836895
6610836892
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis. Mycobacteria Protocols contains a unique collection of both established and recently developed techniques that have been optimized and adapted for use with mycobacteria. Offering an eminently practical introduction for all those carrying out basic or applied research in this important class of bacteria, Mycobacteria Protocols is certain to become the new standard reference in the field, one from which even expert researchers will learn many time-saving techniques.
Other form:Mycobacteria protocols
Standard no.:10.1385/0896034712.

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