Mycobacteria protocols /
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| Imprint: | Totowa, N.J. : Humana Press, ©1998. |
|---|---|
| Description: | 1 online resource (xiv, 472 pages) : illustrations |
| Language: | English |
| Series: | Methods in molecular biology ; v. 101 Methods in molecular biology (Clifton, N.J.) ; v. 101. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11128704 |
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| 050 | 4 | |a QR82.M8 |b M935 1998 | |
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| 245 | 0 | 0 | |a Mycobacteria protocols / |c edited by Tanya Parish and Neil G. Stoker. |
| 260 | |a Totowa, N.J. : |b Humana Press, |c ©1998. | ||
| 300 | |a 1 online resource (xiv, 472 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology ; |v v. 101 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Mycobacteria : bugs and bugbears / Tanya Parish, Neil G. Stoker -- Mycobacteria : general culture methodology and safety considerations / Bryan W. Allen -- Isolation of genomic DNA from mycobacteria / John T. Belisle, Michael G. Sonnenberg -- Nucleic-acid extraction : plasmid/cosmid DNA / Bonnie B. Plikaytis, C. Harold King -- Pulsed field gel electrophoresis for mycobacteria / Wolfgang J. Philipp, Stephen Gordon, Amalio Telenti, Stewart T. Cole -- Extraction of RNA from mycobacteria / Eshwar Mahenthiralingam -- Preparation of cell-free extracts from mycobacteria / Tanya Parish, Paul R. Wheeler -- Preparation of cell-wall fractions from mycobacteria / Gurdyal S. Besra -- Using MycDB on the World-Wide Web / Staffan Bergh, Stewart T. Cole -- Conjugating DNA into mycobacteria / Julie-Ann Gavigan, Carlos Martín -- Electroporation of mycobacteria / Tanya Parish, Neil G. Stoker -- Mycobacteriophages / Gary J. Sarkis, Graham F. Hatfull -- Chemical mutagenesis of mycobacteria / Lucy A. Brooks -- Tn611 transposon mutagenesis in Mycobacterium smegmatis using a temperature-sensitive delivery system / Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giquel, Carlos Martin -- Homologous recombination in Mycobacterium smegmatis : screening methods for detection of gene replacement / Robert N. Husson -- Gene replacement in Mycobacterium smegmatis using a dominant negative selectable marker / Peter Sander, Erik C. Böttger -- Gene replacement in Mycobacterium intracellulare / Britt-Inger Marklund, Richard W. Stokes -- Gene replacement in Mycobacterium bovis BCG / Elizabeth Norman -- The application of bacterial luciferase as a reporter gene in mycobacteria / Stephen Gordon, Gavin Chung, Peter Andrew -- Green fluorescent protein : a tool for gene expression and cell biology in mycobacteria / Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, V. Deretic -- Expression of genes in mycobacteria / Peadar Ó Gaora -- Secretion of mammalian proteins from mycobacteria / Peter J. Murray, Richard A. Young -- Intracellular gene expression : analysis of RNA from mycobacteria in macrophages using RT-PCR / Philip D. Butcher, Joseph A. Mangan, Irene M. Monahan -- Analysis of mycobacterial differential gene expression by RAP-PCR / Joseph A. Mangan, Philip D. Butcher -- Mycobacterial speciation / Francis A. Drobniewski, Anne H. Uttley -- Species identification of mycobacteria using rDNA sequencing / Philip Kirschner, Erik C. Böttger -- Detection of Mycobacterium tuberculosis by a calorimetric polymerase chain reaction / Stuart M. Wilson -- Rapid detection and simultaneous strain differentiation of Mycobacterium tuberculosis complex bacteria by spoligotyping / Henri O. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, Jan D. Embden -- Computer analysis of IS6110 RFLP patterns of Mycobacterium tuberculosis / Herre F. Heersma, Kristin Kremer, Jan D. Embden -- Detection of mutations in mycobacteria by PCR-SSCP (single-strand conformation polymorphism) / Amalio Telenti, Nadine Honoré, Stewart T. Cole -- The use of luciferase-reporter phage for antibiotic-susceptibility testing of mycobacteria / Paul F. Riska, William R. Jacobs. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis. Mycobacteria Protocols contains a unique collection of both established and recently developed techniques that have been optimized and adapted for use with mycobacteria. Offering an eminently practical introduction for all those carrying out basic or applied research in this important class of bacteria, Mycobacteria Protocols is certain to become the new standard reference in the field, one from which even expert researchers will learn many time-saving techniques. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Mycobacteria |v Laboratory manuals. | |
| 650 | 0 | |a Bacterial genetics |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Mycobacterium |x genetics. |
| 650 | 2 | 2 | |a DNA |x physiology. |
| 650 | 2 | 2 | |a Molecular Biology |x methods. |
| 650 | 2 | 2 | |a Mycobacterium |x growth & development. |
| 650 | 7 | |a Bacterial genetics. |2 fast |0 (OCoLC)fst00825221 | |
| 650 | 7 | |a Mycobacteria. |2 fast |0 (OCoLC)fst01031289 | |
| 653 | 0 | 0 | |a mycobacteriaceae |
| 653 | 0 | 0 | |a mycobacterial diseases |
| 653 | 0 | 0 | |a technieken |
| 653 | 0 | 0 | |a techniques |
| 653 | 0 | 0 | |a protocollen |
| 653 | 0 | 0 | |a protocols |
| 653 | 1 | 0 | |a Biological Techniques |
| 653 | 2 | 0 | |a Bacteriology |
| 653 | 2 | 0 | |a Medicine (General) |
| 653 | 1 | 0 | |a Biologische technieken |
| 653 | 2 | 0 | |a Bacteriologie |
| 653 | 2 | 0 | |a Geneeskunde (algemeen) |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 700 | 1 | |a Parish, Tanya. |0 http://id.loc.gov/authorities/names/n98801578 | |
| 700 | 1 | |a Stoker, Neil G. |0 http://id.loc.gov/authorities/names/n98801580 | |
| 776 | 1 | |t Mycobacteria protocols |w (DLC) 98022177 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 101. | |
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| 928 | |t Library of Congress classification |a QR82.M8 M935 1998 |l Online |c UC-FullText |u https://link.springer.com/10.1385/0896034712 |z Springer Nature |g ebooks |i 12530652 | ||